EasyScript® First-Strand cDNA Synthesis SuperMix transgen AE301-02
$50.00
In stock
SKU
AE301-02
Introduction and Features
EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix. • Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis. • The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR. • Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis. • Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA. • cDNA up to 8 kb.
| Applications | Multiple copy gene detection |
| Storage | at -20 ℃ for two years |
| Shipping | Dry ice (-70 ℃) |
Product Composition
| Component | AE301-02 | AE301-03 |
| EasyScript® RT/RI Enzyme Mix | 50 μl | 100 μl |
| 2×ES Reaction Mix | 500 μl | 1 ml |
| Random Primer(N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo(dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
References
1 Dai B, Wang F, Wang Y, et al. Targeting HDAC3 to overcome the resistance to ATRA or arsenic in acute promyelocytic leukemia through ubiquitination and degradation of PML-RARα[J]. Cell Death & Differentiation, 2023.(IF 12.07)
2 Chen H, Qian X, Chen X, et al. Cytoplasmic and nuclear Sw‐5b NLR act both independently and synergistically to confer full host defense against tospovirus infection[J]. New Phytologist, 2021.(IF 8.51)
3 Xiao W, Hu S, Zou X, et al. Lectin receptor-like kinase LecRK-VIII. 2 is a missing link in MAPK signaling-mediated yield control[J]. Plant physiology, 2021.(IF 8.34)
4 Yue X, Song J, Fang B, et al. BcNRAMP1 promotes the absorption of cadmium and manganese in Arabidopsis[J]. Chemosphere, 2021.(IF 7.08)
5 Zheng J, Lu Y, Xiao J, et al. Pan-HDAC inhibitors augment IL2-induced proliferation of NK cells via the JAK2-STAT5B signaling pathway[J]. International Immunopharmacology, 2023.(IF 5.70)
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