Fast PNGase F, Glycerol-free _ 20406ES yeasen
$180.00
In stock
SKU
20406ES20
Product Description
PNGase F is an amidohydrolase that can cleave high-mannose, hybrid, and complex oligosaccharides linked by asparagine, specifically removing N-linked glycans. However, the conventional PNGase F enzymatic reaction often takes several hours to release antibody N-glycans. Due to the preferential release of glycans, incomplete deglycosylation might result in biased outcomes, with the obtained glycan distribution failing to represent the true composition of therapeutic antibodies. Therefore, rapidly obtaining an accurate N-glycan profile is crucial for glycosylation monitoring during the production of antibodies and antibody-fusion proteins used in biotherapeutics. Yeasen Fast PNGase F （ Glycerol-free ） is an optimized recombinant reagent capable of thoroughly and rapidly deglycosylating antibodies, immunoglobulins, fusion proteins, and other glycoproteins within minutes. This enzyme can swiftly and non-selectively remove all N-glycans, enabling direct downstream chromatography or mass spectrometry analysis. Yeasen Fast PNGase F
（ Glycerol-free ） simplifies the experimental procedure while reducing experimental time without compromising sensitivity and reproducibility.
Features
- Fast
- —
- Complete and rapid deglycosylation in a few minutes.
- High purity—No protease, glycosidase contamination, purity ≥95%.
- Non-selective
- —
- Quickly and without preference remove all N-glycans.
- Good compatibility
- —
- Directly used for downstream chromatography or MS spectrometry analysis.
Applications
- Glycan Sequencing
- Glycoprotein Analysis
- Recombinant Glycoprotein Expression
Specifications
| Source | Yeast recombinant expression |
| Storage Buffer | 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA |
Additional Information
- English synonym
- Fast PNGase F
- （
- Glycerol-free
- ）
Components
- Components No.
- Name
- 20406ES20
- 20406ES50
- 20406-A
- Fast PNGase F
- 20
- μL
- 50
- μL
- 20406-B
- Fast PNGase F Buffer
- （
- 5×
- ）
- 80
- μL
- 200
- μL
Storage
The product can be stored at 2 ~ 8℃ for one year, do not freeze
Figures
Figure 1. Two -step enzymatic cleavage effect (protein substrate) 1: Protein Marker(Cat#20350ES) 2: Supplier N* + substrate 3: Supplier N* + substrate 4: Yeasen Fast PNGase F + substrate 5: Yeasen Fast PNGase + substrate F 6: Substrate 7: Yeasen Fast PNGase F 8: Supplier N* 2 Two -step enzymatic cleavage effect (antibody) 1: Protein Marker(Cat#20350ES) 2: Supplier N* + antibody 3: Supplier N* + antibody 4: Yeasen Fast PNGase F + antibody 5: Yeasen Fast PNGase F + antibody 6: Antibody 7: Yeasen Fast PNGase F 8: Supplier N* Related Reading Fast PNGase F, Completing Deglycosylation in Just 10 Minutes
Safety Data Sheet
- 20406_MSDS_HB260122_EN.PDF
Manuals
- 20406_Manual_HB240524_EN.pdf
Citations & References
- 1. Wang N, Ji W, Jiao H, et al. A MERS-CoV-like mink coronavirus uses ACE2 as an entry receptor. Nature. 2025;642(8068):739-746. doi:10.1038/s41586-025-09007-w(IF:48.5)
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