Mouse CD73/NT5E Protein, His Tag yeasen
$170.00
In stock
SKU
93993ES25
Specifications
| Source | Mouse CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Trp29-Lys549.[Accession | Q61503] |
| Source | The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Purity | > 95% as determined by Tris-Bis PAGE and HPLC |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 5% trehalose is added as protectant before lyophilization. |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Activity | ELISA Data: Immobilized Mouse CD73, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. Bioactivity Data: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The specific activity is >10000 pmol/min/μg |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Notes
1. Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
