HEK293 Host Cell Residue DNA Size Analysis Kit _ 41316ES yeasen
$4,775.00
In stock
SKU
41316ES70
Product Description
The HEK293 Host Cell Residue DNA Size Analysis Kit is designed for the quantitative analysis of HEK293 residual DNA fragments of various lengths in intermediates, semi-finished products, and final products of biological preparations. This kit utilizes the qPCR fluorescence probe principle to specifically and rapidly detect HEK293 DNA residues both below and above 200 base pairs, with a quantification limit as low as 10 fg/μL. It also includes the HEK293 DNA Control (DNA quantitative reference). This kit can be used in conjunction with the company's magnetic bead-based residual DNA sample preparation kits (Cat#18461ES). Validation Report
Features
- Compliance with regulations: the products are fully validated according to the requirements of Chp, USP, ICH, etc., and their performance is in line with Chinese and foreign regulations and standards;
- Guarantee of quality: the raw materials of the kits are all self-developed, and the qPCR Mix and other enzyme products are produced in an ultra-clean enzyme factory;
- High sensitivity: the limit of quantification can reach
- 10
- fg/μL level;
- High precision: high intra-batch repeatability and low inter-batch variation;
- Strong exclusivity: specific detection of residual DNA in
- HEK293
- cells without interference from other exogenous genomic DNA
- Strong anti-interference: adding UDG enzyme, digesting routine product aerosol contamination, preventing non-specific amplification
Applications
- Residual
- HEK293
- Host Cell DNA Size
- test in biological products
Specifications
- Sensitivity
- 10 fg/μL (range 30 fg/μL~300 pg/μL)
- Assay Time
- ~1.5 hours
- Assay Principle
- Fluorescent probe qPCR method
- Required Instruments
- Real-time fluorescence quantitative PCR system
Components
- Components No.
- Name
- 41316
- ES
- 70-EN
- 41316
- ES
- 74-EN
- 41316-A
- HEK293 qPCR Mix
- 0.
- 75
- mL×
- 4
- 1
- 5
- mL×
- 4
- 41316-B1
- HEK293 Primer&
- P
- robe Mix-82
- 250 μL
- 500 μL
- 41316-B2
- HEK293 Primer&
- P
- robe Mix-133
- 250 μL
- 500 μL
- 41316-B3
- HEK293 Primer&
- P
- robe Mix-227
- 250 μL
- 500 μL
- 41316-B4
- HEK293 Primer&
- P
- robe Mix-515
- 250 μL
- 500 μL
- 41316-C
- DNA Dilution Buffer
- 1.8
- mL×
- 2
- 1.8
- mL×
- 4
- 41316-D
- HEK293 DNA Control
- (
- 30
- ng/μL
- )
- 25 μL
- 50 μL
Storage
This product should be stored at -25~-15℃ for 2 years. Both 41316 -A and 41316 -B 1&B2&B3&B4 all should be stored protected from light
Figures
Limit of quantification The primer probes for the four fragments, 82bp, 133bp, 227bp and 515bp, were used to detect HEK293 DNA at 30fg/μL, 20fg/μL, 10fg/μL (i.e. 0.01pg/μL), with 10 replicates for each concentration, respectively. The results showed that at concentrations of 10fg/μL (i.e., 0.01pg/μL) and above, the CVs of the four fragments were <20%, and the backcalculated ratios were all between 70% and 130%, so the limits of quantification of the four fragments of the HEK293 Residual DNA Fragmentation Analysis Kit were all 10fg/μL (i.e., 0.01pg/μL). Repetition number T est item HEK293 DNA (fg /μL ) 82bp 133bp 227bp 515bp Quantity Return ratio Quantity Return ratio Quantity Return ratio Quantity Return ratio 1 0.011 110% 0.009 90% 0.010 95% 0.009 88% 2 0.009 91% 0.009 93% 0.009 86% 0.009 93% 3 0.011 107% 0.009 95% 0.009 87% 0.009 88% 4 0.011 112% 0.010 97% 0.009 92% 0.009 93% 5 0.011 108%
0.010 95% 0.009 87% 0.010 99% 6 0.009 94% 0.010 102% 0.009 90% 0.010 98% 7 0.010 97% 0.009 93% 0.009 87% 0.009 87% 8 0.010 100% 0.011 111% 0.010 101% 0.009 91% 9 0.010 102% 0.010 102% 0.008 83% 0.009 94% 10 0.010 100% 0.009 90% 0.008 84% 0.010 101% A verage value 0.010 0.090 0.008 CV% 7% 8% 14% Table 1. Limit of quantification (LOQ) detection results of four fragments of 82bp, 133bp, 227bp and 515bp
Related Blog
- Advancing Biopharmaceutical Quality Control: Unveiling the Science of HEK293 Residual Detection
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