SV40LTA&E1A Residue DNA Detection Kit _ 41310ES yeasen
$955.00
In stock
SKU
41310ES50
Product Description
This kit is designed for the quantitative detection of residual host cell DNA—specifically SV40 Large T Antigen (LTA) and Adenovirus E1A sequences—in biopharmaceutical products derived from host cells such as HEK293T. Utilizing probe-based real-time PCR (qPCR), the assay enables highly specific and rapid quantification of SV40 LTA and E1A residual DNA, with a limit of detection as low as 10¹ copies/μL. The kit is fully compatible with Yeasen’s Magnetic Bead–Based Residual DNA Sample Preparation Kits (Cat# 18461ES) for streamlined sample processing.
Features
- Complies with regulations
- – Fully validated; validation reports available.
- High-quality reagents
- – All components in-house developed; enzymes made in ultra-clean facility.
- Highly sensitive
- – Detection down to 10¹ copies/μL.
- Highly reproducible
- – Low within-run and between-run variability.
- Highly specific
- – Detects only SV40 LTA and E1A DNA—no cross-reactivity.
- Built-in control
- – Internal Control (IC) flags sample or reaction issues.
Components
- Components No.
- Name
- 41310ES50(50 T)
- 41310ES60(100 T)
- 41310-A
- SV40LTA&E1A qPCR
- Mix
- 0.75
- mL
- 1.5
- mL
- 41310-B
- SV40LTA&E1A
- Primer&Probe
- M
- ix
- 200
- μL
- 400
- μL
- 41310-C
- DNA
- Dilution
- Buffer
- 2
- ×
- 1.8
- mL
- 4
- ×
- 1.8
- mL
- 41310-D
- SV40LTA&E1A
- DNA
- Control
- (nonliner)
- 25
- μL
- 50
- μL
- 41310-E
- SV40LTA&E1A
- DNA
- Control
- (line
- ar)
- 25
- μL
- 50
- μL
- 41310-F
- IC
- 50
- μL
- 100
- μL
- *IC
- Internal control.
Storage
This product should be stored at -25~-15 ℃ for 2 years. Both 41310-A and 41310-B should be stored protected from light. Applicable Instrument Models Include but not limited to: Bio-Rad: CFX96 Optic Module. Thermo Scientific: ABI 7500; ABI Quant Studio 5.
Figures
1. Detection Range SV40LTA: Linear range = 1.8 × 10¹ ~ 1.8 × 10⁵ copies/μL, R² = 1.000, amplification efficiency = 100.25%, CV < 15% across all concentrations. E1A: Linear range = 1.8 × 10¹ ~ 1.8 × 10⁵ copies/μL, R² = 1.000, amplification efficiency = 100.25%, CV < 15% across all concentrations.
Figure 1. SV40LTA standard curve (left) and amplification plot (right)
Figure 2. E1A standard curve (left) and amplification plot (right) 2. Limit of Quantitation (LOQ) SV40LTA & E1A DNA at concentrations of 18, 9, 4.5, 2.25, and 1.8 copies/μL were tested in 10 replicates each. At ≥4.5 copies/μL, CVs were <20%, establishing the LOQ as 4.5 copies/μL. 3 . qPCR detection of SV40LTA (lift) and E1A(right) at 4.5 copies/μL Replicate Analyte SV40LTA (copies/μL) E1A (copies/μL) Quantity Recovery Quantity Recovery 1 4.66 103.61% 4.87 108.21% 2 4.33 96.29% 4.15 92.26% 3 4.49 99.72% 4.63 102.88% 4 5.08 112.97% 4.86 107.99% 5 6.26 139.11% 5.67 125.93% 6 5.46 121.26% 4.97 110.42% 7 4.79 106.34% 5.50 122.22% 8 5.57 123.83% 5.00 111.20% 9 5.09 113.18% 5.55 123.28% 10 4.62 102.62% 4.97 110.43% Mean 5.04 5.02 CV% 11.73% 9.13%
Safety Data Sheet
- 40310
- _MSDS_HB
- 260
- 203
- _EN.PDF
Manuals
- 40310
- _Manual_Ver.E
- N
- 20260
- 203
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