Anti-rabbit Nanobody-Tn5 _ 12594ES yeasen
$205.00
In stock
SKU
12594ES12
Product Description
Anti-rabbit Nanobody-Tn5 is a recombinant Tn5 transposase fused with nanobody which can specific bind with primary antibodies from rabbit.
Product Information
- Catalog No.
- 12594ES04/12594ES12/12594ES48
Specifications
- 4T/ 12T/ 48T
- Product Components
- Component name
- 12594ES04
- 12594ES12
- 12594ES48
- 12594-A
- Anti-rabbit Tn5
- 4 μL
- 12 μL
- 48 μL
- 12594-B
- Annealing Buffer
- 1 mL
- 1mL
- 12594-C
- Assemble Buffer
- 4 μL
- 12 μL
- 48 μL
Shipping and Storage
Stored at -85° ～ -65℃ for one year.
Figures
H3K4me1: H3K4me3 H3K9me3 H3K27ac H3K27me3 Control: Conventional pA/G-Tn5, Cut&Tag Multi Cut&Tag: Nanobody Tn5 Instruction Take high-throughput sequencing library preparation as an example, be sure to read the instructions carefully before use. 1. Adapter preparation 1) Reference oligo name and sequence of Illumina platform: Oligo A-1：5'-AATGATACGGCGACCACCGAGATCTACACATAAGGCTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3' Oligo A-2：5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′ Oligo B：5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′ Oligo C：5'-phos-CTGTCTCTTATACACATCT-3' 2) Dissolve the Oligo A, Oligo B, and Oligo C to 200 μM. 3) The following reaction systems are formulated respectively:
Components
- Reaction 1
- Oligo B （200 μM）
- 10 μL
- Oligo C （200 μM）
- 10 μL
- total
- 20 μL
- Reaction 2
- Oligo A-1 or A-2 （200 μM）
- 10 μL
- Oligo C （200 μM）
- 10 μL
- total
- 20 μL
- 4) Mix reaction 1 and 2 together, and briefly centrifuge to bring the solution back to the bottom of the tube. Place in the PCR instrument, set up the following program and run it: hot lid at 105°C,and then heat at 94℃ for 2 min. After the time was up, the reaction procedure was stopped without opening the hot lid.The PCR tube was naturally cooled in the PCR instrument for 2 h, and then placed at 4℃ for 5 min.
- 5) After the reaction, mix the equal volumes of Reaction 1 and Reaction 2. Named ad Adapter Mix and stored from – 25 to – 15℃.
- 2. for transposon generation
- 1) To configure the following reaction system:
- volume (μL)
- Anti-rabbit Tn5
- 10
- Adapter mix (50 μM)
- 0.5
- Assemble Buffer
- 0.5
- 2) Reaction conditions: use the pipette to gently blow and mix well. Perform the reaction at 25℃ for 1 h (hot lid off). The products of reaction can be directly applied to the library preparation or stored from -25 to -15℃.
Notes
1. Transposase is temperature-sensitive, so it is recommended to complete the adapter embedding as soon as possible. The generated transposon can be stored at-25~ -15℃, with a validity period of 1 year. 2. This product is used for scientific research purposes only. 3. For your safety and health, please wear laboratory clothes and wear disposable gloves.
Safety Data Sheet
- 12594_MSDS_HB251216_EN.PDF
Manuals
- 12594_Manual_Ver.EN20251216.pdf
MDSD
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