Human CD74/DHLAG (His Tag) yeasen
$140.00
In stock
SKU
93769ES25
Product Description
CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading . Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface. CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the upregulation of angiogenic factors in endometrial stromal cells. MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation. CD74 is upregulated on nonimmune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques . It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis.
Specifications
| Synonyms | CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia-GAMMA ; II |
| Source | Recombinant Human CD74/DHLAG Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Gln73-Met232. |
| Molecular Weight | Approximately 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result. |
| Purity | > 95% as determined by SDS-PAGE. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Endotoxin | < 1.0 EU per 1μg of the protein by the LAL method. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Accession | NP_004346.1 |
| Activity | ELISA Data: Immobilized Human CD74 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year. After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days. It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Notes
1. Avoid repeated freezing and thawing. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for scientific research purposes only.
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