Hieff NGS™ OnePot Evo DNA Library Prep Kit (Enzymatic, For FFPE, V4) _ 12193ES yeasen
$135.00
In stock
SKU
12193ES08
Product Description
Hieff NGS™ OnePot Evo DNA Library Prep Kit is a next-generation enzymatic library preparation kit compatible with both Illumina and MGI high-throughput sequencing platforms. Compared to traditional library construction methods, this kit replaces time-consuming and instrument-dependent sonication with a high-quality, low-bias fragmentation enzyme. It uniquely integrates the fragmentation, end repair, and dA-tailing steps into a single reaction, significantly reducing hands-on time, cost, and workflow complexity. The ligation module features pre-mixed enzyme and buffer for streamlined operation, making the kit highly suitable for automation. Building upon the previous version, this updated formulation further reduces GC bias, enhances end-repair and dA-tailing efficiency, and improves reagent stability. It also incorporates a newly optimized ligase for superior adapter ligation performance. The kit is fully compatible with standard Illumina or MGI adapters and primers. Ideal for whole-genome sequencing of animals, plants, microbes, and challenging clinical samples.
Features
- s
- Wide input range:
- Compatible with 5 ng–1 µg of genomic DNA, full-length cDNA, or other double-stranded DNA
- High-quality fragmentation enzyme:
- Enables random dsDNA cleavage with minimal sequence bias, integrating fragmentation, end repair, and dA-tailing in a single step
- High-fidelity, high-efficiency PCR enzyme:
- Maximizes library yield and complexity while preserving sequence accuracy
- FFPE-compatible:
- Delivers robust performance with degraded or formalin-fixed DNA samples
- Rigorous quality control:
- Each production batch undergoes stringent performance validation and stability testing
Specifications
- Cat.N
- o
- 12193ES08 / 12193ES24 / 12193ES96
- Size
- 8 T / 24 T / 96 T
Components
- Components No.
- Name
- 12193ES08
- 12193ES24
- 12193ES96
- 12193-A
- Smearase™
- Buffer
- 40 μL
- 120
- μL
- 480
- μL
- 12193-B
- Smearase™
- Enzyme
- 80 μL
- 240
- μL
- 960
- μL
- 12193-C
- Rapid Ligase Master Mix
- 280 μL
- 840
- μL
- 4×840
- μL
- 12193-D
- 2× Ultima HF Amplification Mix
- 200 μL
- 600
- μL
- 3×800 μL
- [Note]:
- The kit components are compatible with both Illumina &MGI sequencing platform, if the complete adapter was used, Hieff NGS Primer Mix (Yeasen Cat#12190 or Cat#12191) in needed.
Storage
This product should be stored at -25~-15℃ for one year..
Applications
- DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing;
Figures
1. Workflow
Figure 1. The workflow of OnePot Pro DNA Library Prep Kit 2. Flexible and High-Efficiency Fragmentation
Figure 2. Fragment size distribution of 250 ng human gDNA using the 12193 fragmentation module. Calf gDNA (250 ng) was fragmented at 30°C ~ 35°C for 5 min, 10, 15, 20, 30. The distribution of recovered insert sizes is shown, demonstrating stable and uniform fragmentation across the 5 – 3 0 minute range. Different DNA samples were fragmented with 12 194 under identical conditions. The libraries showed consistent sizes (~450 bp / ~320 bp) with good uniformity across species.
Figure 3. Comparison of soft-clipping rates (%) and false chimeric read rates (%) across human gDNA, ctDNA, and FFPE DNA samples. The results show that 12194 exhibits lower or comparable soft-clipping rates (%) and false chimeric rates(%). 3. Strong Stability
Figure 4. Library yield stability after storage at 4 ° C and 25 ° C for 4 weeks. No decrease in library yield was observed after 4 weeks of storage at 4 ° C and 25 ° C, highlighting the kit ’ s suitability for automation.
Manuals
- 12193_Manual_HB20260430
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