2×Hieff™ Multiplex PCR Master Mix for NGS _ 13606ES yeasen
$285.00
In stock
SKU
13606ES03
Product Description
The Hieff™ Multiplex PCR Master Mix for NGS is a ready-to-use 2× premix developed for multiplex PCR applications. It contains a hot-start, multiplex-optimized Taq DNA polymerase blend and a proprietary amplification buffer system. The master mix enables rapid and convenient multiplex PCR and supports efficient amplification of targets with GC content ranging from 25% to 75%. It delivers high uniformity, specificity, and sensitivity across multiple amplicons. This reagent is suitable for multiplex amplification of 30–300 amplicons of different sizes and can also be used for capture-based assays involving thousands or more amplicons. It is widely applicable in animal and plant breeding, forensic identification, genetic disease testing, food safety analysis, and related fields.
Features
- Easy to operate:
- Ready-to-use 2× premixed solution
- Broad Compatibility:
- Suitable for complex samples, GC-rich/poor systems(25-75%), and multiplex amplification.
- Excellent performance:
- High uniformity, specificity, and sensitivity; supports 30–300 multiplex amplicons or enrichment of thousands of targets.
Components
- Components No.
- Name
- 1
- 3606
- ES0
- 3
- 1
- 3606
- ES
- 08
- 1
- 3606
- ES
- 50
- 13606ES65
- 13606
- 2
- ×
- Hieff™
- Multiplex PCR Master Mix
- for NGS
- 1
- m
- L
- 5
- m
- L
- 5
- 0
- m
- L
- 6
- 5 mL
Storage
This product should be stored at -25~-15℃ for 2 year s
Applications
- Targeted gene panels:
- Amplify disease-related genes (e.g., cancer, inherited disorders).
- Direct blood PCR:
- No DNA extraction needed.
- Forensic
- identification
- Type STRs/SNPs for identity.
- Agricultural breeding:
- Detect trait-associated markers (e.g., SNPs, indels).
Figures
1. High A mplification E fficiency NO. DNA Concentration(ng/uL ） Supplier B* (ng/uL ） Yeasen (ng/uL ） SNP-1 34.8 4.66 41.2 SNP-2 67.8 5.5 46.8 SNP-3 46.6 8.28 44.4 SNP-4 45.8 8.54 44 SNP-5 66.4 7.44 43.2 SNP-6 69.2 6.62 44.4 SNP-7 102 10.8 38 SNP-8 70.6 9.86 44
Figure 1. Multiplex amplification test using 13606ES for 300-plex SNP genotyping. Amplicons (100–200 bp) show high specificity and uniformity across all targets.
Safety Data Sheet
- 1
- 3606
- _MSDS_HB
- 2
- 60122
- _EN.PDF
Manuals
- 1
- 3606
- _Manual_Ver.E
- N
- 202
- 60127
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