The Hieff NGS™ C37P3 ds-cDNA Synthesis Kit (gDNA digester plus) is a cDNA synthesis kit for RNA sequencing libraries for the Illumina™/MGI™ sequencing platforms, which includes gDNA removal reagents, RNA reverse transcription reagents, and ds-cDNA synthesis reagents. All reagents provided in this kit have undergone strict quality control and functional validation to ensure the stability and reproducibility of library construction to the greatest extent.
Features
Fast
: Rapid cDNA Synthesis. No purification of the second-strand product. gDNA digester and cDNA synthesis only takes 92 minutes.
Compatible
: Compatible with 100 pg - 10 ng total RNA, as well as nucleic acids co-extracted from DNA and RNA.
This product should be stored at -25~-15℃ for 1 year.
Figures
RNA input 100 pg-100 ng RNA: 100 pg-100 ng ds-cDNA Synthesis ds-cDNA Synthesis (Cat#13488ES) ds-cDNA Synthesis with gDNA digester (Cat#13488ES) ds-cDNA Synthesis time 53 min 92 min Library Prep Flash DNA Library Prep (Cat#12316ES) Flash DNA Library Prep (Cat#12316ES) Library Prep Time 75 min Species compatibility RNA (prefer) or DNA/RNA mixture RNA or DNA/RNA mixture