Hieff NGS™ ds-cDNA Synthesis Kit _ 13488ES yeasen
$125.00
In stock
SKU
13488ES08
Product Description
The Hieff NGS™ ds-cDNA Synthesis Kit is a cDNA synthesis kit for RNA sequencing libraries on the Illumina™ /MGI™ sequencing platforms, containing efficient RNA reverse transcription reagents and conventional ds-cDNA synthesis reagents. It can be connected to the Hieff NGS™ OnePot Flash DNA Library Prep Kit (Yeasen Cat#12316ES) for subsequent library construction. All reagents provided in this kit have undergone strict quality control and functional validation, ensuring the stability and reproducibility of library construction to the greatest extent.
Features
- Fast: Rapid cDNA Synthesis. No purification of the second-strand product. cDNA synthesis only takes 53 minutes.
- Compatible: Compatible with 100 pg - 500 ng total RNA.
- High-quality: Upgraded reverse transcription module, excellent pathogen detection, and strict background bacteria quality control.
Applications
- ·
- Double-stranded cDNA Synthesis
- ·
- Metatranscriptome Detection
- ·
- Pathogen RNA library Prep
Specifications
| Cat.No. | 13488ES08 / 13488ES24 / 13488ES96 |
| Size | 8 T / 24 T / 96 T |
Components
- Components No.
- Name
- 13488ES08
- 13488ES24
- 13488ES96
- 13488-A
- Random Primer
- 8
- μ
- L
- 24
- μ
- L
- 212
- μ
- L
- 13488-B
- 1st Reaction Buffer
- 64
- μ
- L
- 192
- μ
- L
- 1267
- μ
- L
- 13488-C
- 1st Strand Enzyme Mix
- 16
- μ
- L
- 48
- μ
- L
- 320
- μ
- L
- 13488-D
- 2nd Reaction Buffer
- 56
- μ
- L
- 168
- μ
- L
- 1034
- μ
- L
- 13488-E
- 2nd Strand Enzyme Mix
- 24
- μ
- L
- 72
- μ
- L
- 473
- μ
- L
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.
Figures
RNA input 100 pg-100 ng RNA: 100 pg-100 ng ds-cDNA Synthesis ds-cDNA Synthesis (Cat#13488ES) ds-cDNA Synthesis with gDNA digester (Cat#13488ES) ds-cDNA Synthesis time 53 min 92 min Library Prep Flash DNA Library Prep (Cat#12316ES) Flash DNA Library Prep (Cat#12316ES) Library Prep Time 75 min Species compatibility RNA (prefer) or DNA/RNA mixture RNA or DNA/RNA mixture
Safety Data Sheet
- 13488ES-MSDS-HB240223.PDF
Manuals
- 13488_Manual_Ver. EN20250205_EN.pdf
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