Endonuclease VIII(10 U/μL) _ 14536ES yeasen
$65.00
In stock
SKU
14536ES80
Product Description
Endonuclease VIII is a DNA repair enzyme that possesses both N-glycosylase activity and AP-lyase activity. The N-glycosylase activity can release damaged pyrimidine bases from double-stranded DNA, creating an abasic (AP) site. The AP-lyase activity then cleaves the phosphodiester bond at the AP site, resulting in a gap with a 5' phosphate and a 3' phosphate. The damaged bases that Endonuclease VIII recognizes and excises include: urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methoxyhydantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymidine, and methylhydroxypropanone.
Features
- No residual of exonucleases, endonucleases, or RNases
- High-efficiency removal of damaged bases
Applications
- Single-cell gel electrophoresis (Comet assay)
- Alkaline elution
- Alkaline unwinding
Specifications
- S
- ource
- E. coli
- Molecular
- W
- eight
- 29
- KD
- a
- Concentration
- 10 U/
- μL
- Unit
- D
- efinition
- One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 bp oligonucleotide duplex containing one AP site* in a 10 µ
- L
- total reaction system with 10 pmol of fluorescently labeled oligonucleotide duplex in 1X Endonuclease VIII
- R
- eaction buffer at 37°C within 1 hour
- I
- nactivation
- C
- onditions
- I
- ncubate at 75°C for 10 minutes
Components
- No.
- Name
- 14536ES80
- 14536ES90
- 14536-A
- Endonuclease VIII(10 U/μL)
- 100
- μL
- 500
- μL
- 14536-B
- 10 × Endonuclease VIII Reaction Buffer
- 1.5
- mL
- 1.5
- mL
Shipping and Storage
The products should be stored at - 2 5℃ ~ - 1 5℃ for 1 year.
Figures
Figure1. Base excision repair effect comparison Note: 20 pmol of oligonucleotides were catalyzed by Endonuclease VIII from Yeasen and SupplierA, respectively, in a 20 µL reaction system with the addition of 5~40 U of Endonuclease VIII and equivalent enzyme activity of UDG (Cat#10303ES), and incubated at 3 7 ℃ for 1 hour. The results of agarose gel electrophoresis showed that the base excision effects of Yeasen 's Endonuclease VIII and Supplier A's were consistent.
Safety Data Sheet
- 14536_MSDS_Ver.EN20241210.pdf
Manuals
- 14536_Manual_Ver.EN20241210.pdf
Reviews
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