Klenow Fragment(3'→5' exo-) (5 U/μL) _ 14460ES yeasen
$45.00
In stock
SKU
14460ES70
Product Description
Klenow (3' → 5'exo-) DNA polymerase is a proteolytic product of E. coli DNA polymerase I, retaining the DNA polymerase activity of DNA polymerase I but losing the 5' → 3' exonuclease activity. This enzyme has further removed the 3' → 5' exonuclease activity thro ugh mutation. It can be used for DNA labeling with random priming and DNA sequencing by the dideoxy method, among other applications.
Features
- No nickase or exonuclease residue
- Low residual host DNA
- High amplification efficiency
Applications
- Random primer labeling
- Sanger dideoxy DNA sequencing
- Synthesis of the second strand of cDNA
- Synthesis of the second strand in mutagenesis reactions
Specifications
| Molecular Weight | 68.1 KDa |
| Concentration | 5 U/μL |
| Unit Definition | One unit is defined as the amount of enzyme that incorporates 10 nmol of dNTP into acid-insoluble material within 30 minutes at 37°C |
Components
- No.
- Name
- 14460ES70
- 14460ES80
- 14460ES90
- 14460-A
- Klenow Fragment(3'
- →
- 5' exo-) (5 U/
- μ
- L)
- 40 μL
- 200 μL
- 1 mL
- 14460-B
- 10×Klenow Fragment (exo-) Buffer
- 250 μL
- 1.25 mL
- 5×1.25 mL
Shipping and Storage
This product should be stored at -25 ~ -15 ℃ for 2 years.
Figures
Figure1. Residual E.coli genomic DNA detection Note:The residual E. coli genomic DNA in three batches of Klenow Fragment (3' → 5' exo-) (Cat#14460) is below 1 copy/50 U. 2 . Product Stability Testing Note: Klenow Fragment (3' → 5' exo-) from Yeasen and Supplier A was stored at 37 ° C for 7 and 14 days to assess residual enzyme activity. The results indicate that the residual activity of Yeasen 's Klenow Fragment (3' → 5' exo-) (Cat#14460) after 14 days at 37 ° C is notably higher than that of the Supplier A 3 . NGS library yield Note: Using the same amount of Klenow Fragment (3' → 5' exo-) (Cat#14460) from Yeasen and Supplier A for NGS library construction, the results show that the library yield with Yeasen 's Klenow Fragment (3' → 5' exo-) (Cat#14460) at both 5 U and 10 U input is h igher than that of Supplier A.
Safety Data Sheet
- 14460_MSDS_Ver.EN20241210.pdf
Manuals
- 14460_Manual_Ver.EN20241210.pdf
Reviews
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