Klenow Fragment (5 U/μL) _ 14458ES yeasen
$45.00
In stock
SKU
14458ES70
Product Description
Klenow Fragment is a proteolytic product of E. coli DNA Polymerase I, retaining the DNA polymerase activity and 3'→5' exonuclease activity of DNA Polymerase I, but lacking the 5'→3' exonuclease activity. It can be used for random priming DNA labeling and the synthesis of the second strand of cDNA.
Features
- No residual nucleases or RNases
- High amplification efficiency
Applications
- DNA sequencing by the Sanger dideoxy method
- Second strand cDNA synthesis
- DNA labeling using random primers
Specifications
| Source | E. coli |
| Molecular Weight | 68.2 kDa |
| Concentration | 5 U/μL |
| Unit Definition | One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C |
Components
- Components No.
- Name
- 14458ES70
- 14458ES80
- 14458-A
- Klenow Fragment (5 U/μL)
- 40 μL
- 200 μL
- 14458-B
- 10×Klenow Fragment Buffer
- 1.25 mL
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Figures
Figure1. Detection of E. coli genomic DNA residue Note: The residual E. coli genomic DNA in three batches of Klenow Fragment (Cat#14458) is below 1 copy (at 50 U). 2 . Product Stability Testing Note: The Klenow Fragment enzyme from Yeasen (Cat#14458), along with its supplier A, was subjected to a stability test at 37°C for durations of 7 and 14 days to measure the residual enzymatic activity. The findings indicate that Yeasen's Klenow Fragment maintained its activity without any significant decline after a week at 37°C, and retained approximately 90% of its initial activity after two weeks.
Safety Data Sheet
- 14458_MSDS_Ver.EN20241210.pdf
Manuals
- 14458_Manual_Ver.EN20241210.pdf
Reviews
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