T4 polynucleotide Kinase(10 U/μL) _ 12902ES yeasen
$40.00
In stock
SKU
12902ES76
Product Description
T4 Polynucleotide Kinase (T4 PNK) is a 5'-hydroxyl kinase that facilitates the transfer of the γ-phosphate from ATP to the 5'-hydroxyl termini of oligo- or polynucleotide chains, including double-stranded and single-stranded DNA or RNA, as well as to 3'-monophosphate nucleotides. This transfer is reversible. T4 PNK also possesses 3'-phosphatase activity, which dephosphorylates 3'-phosphorylated oligonucleotide termini, deoxy 3'-monophosphate nucleotides, and deoxy 3'-diphosphate nucleotides. In the presence of ADP, T4 PNK can act as a 5'-phosphatase, promoting the exchange of 5'-phosphate groups on 5'-phosphorylated oligo-or polynucleotides with ATP. This enzyme is widely used for the construction of DNA libraries and for end labeling to generate probes.
Applications
- Phosphorylation of the 5' ends of DNA or RNA for subsequent ligation
- Terminal labeling of DNA or RNA to serve as probes and for sequencing purposes
- 5' phosphorylation of mononucleotides with a phosphorylated 3' end to prepare pNp substrates for attachment to the 3' ends of DNA or RNA
- Labeling the 5' ends of oligonucleotides that possess a 3' phosphate group
Specifications
| Source | Bacteriophage T4 |
| Molecular Weight | 36.78 kDa |
| Concentration | 10 U/μL |
| Unit Definition | One unit is defined as the the amount required to catalyze the incorporation of 1 nmol of [γ-32P] ATP into a reaction within 30 minutes at 37°C |
Components
- Components No.
- Name
- 12902ES76
- 12902ES86
- 12902ES92
- 12902-A
- T4 polynucleotide Kinase(10 U/μL)
- 50 μL
- 250 μL
- 1 mL
- 12902-B
- 10×T4 PNK Buffer
- 500 μL
- 1 mL
- 2×1 mL
- [Note]:
- The 10×T4 PNK Buffer does not contain ATP; customers need to add it themselves, with a reference final concentration of 1 mM, or use the T4 DNA ligase buffer.
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Safety Data Sheet
- 12902_MSDS_Ver.EN20241210.pdf
Manuals
- 12902_Manual_Ver.EN20250729.pdf
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