UCF.ME™ Thermostable RNase H (5 U/μL) _ 14545ES yeasen
$110.00
In stock
SKU
14545ES72
Product Description
UCF.ME TM Thermostable RNase H, a thermostable ribonuclease endonuclease, exhibits specific activity at elevated temperatures, targeting the phosphodiester bonds within RNA sequences of RNA:DNA hybrid structures while preserving the integrity of DNA sequences. Furthermore, it does not degrade single-stranded or double-stranded RNA or DNA substrates. Despite sharing similar ribonuclease endonuclease characteristics with its E. coli counterpart, the thermostable enzyme demonstrates enhanced activity at higher temperatures, with optimal functionality exceeding 65 o C. This attribute enables UCF.ME TM Thermostable RNase H to be utilized in high-temperature experimental settings, ensuring heightened sensitivity and minimizing nonspecific hybridization backgrounds. Yeasen offers comprehensive solutions for experiments . Explore our related products: Ultra-Clean UCF.ME® Thermostable RNase H: Precise Cutting for More Efficient Experiments!
Features
- High activity and excellent batch-to-batch consistency.
- Low host gDNA residue: <0.02 copies/U.
- Free of exonuclease, nickase, and RNase residue.
- Superior stability: No significant decrease in enzyme activity after 32 days at 4
- o
- C, 16 days at 25
- o
- C, or 7 days at 37
- o
- C.
Applications
- rRNA removal
- Detection of mRNA capping efficiency
- Removal of mRNA poly
- (A) hybridized to poly
- (dT)
- Elimination of mRNA during cDNA second-strand synthesis.
- Enhancement of amplification efficiency in isothermal amplification experiments
Specifications
| Concentration | 5 U/μL |
| Cat.No. | 14545ES72 / 14545ES82 |
| Size | 250 U / 1250 U |
| Unit Definition | One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 picomoles of a fluorescently labeled 25 base pair RNA-DNA hybrid in a total reaction volume of 50 µl in 20 minu tes at 50 o C Reaction conditions ≥50 o C |
Components
- Components No.
- Name
- 14
- 545
- ES
- 72
- 14
- 545
- ES
- 82
- 14
- 545
- -A
- UCF.ME
- TM
- Thermostable RNase H (5 U/μL)
- 5
- 0
- μL
- 25
- 0
- μL
- 14
- 545
- -B
- 10×RNase H Reaction Buffer
- 1 mL
- 5×1 mL
Shipping and Storage
This product should be stored at -25 ~ -15 o C for 1 year.
Figures
1. Activity Detection Results of UCF.ME TM Thermostable RNase H. Note: Reaction conditions: 50°C for 20 minutes; RNA:DNA substrate - 20 pmol 2 . Host gDNA Residue Results of UCF.ME TM Thermostable RNase H. 3 . Free of exonuclease, nickase, and RNase residue. 4 . Accelerated Stability Results of UCF.ME TM Thermostable RNase H
Safety Data Sheet
- 1
- 4
- 545
- _MSDS_
- Ver.EN202
- 50227
Manuals
- 1
- 4
- 545
- _Manual_Ver.EN202
- 50121
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
