T7 Endonuclease I (10 U/µL) _ 14548ES yeasen
$65.00
In stock
SKU
14548ES72
Product Description
T7 Endonuclease I is encoded by gene 3 of bacteriophage T7. It recognizes and cleaves mismatched DNA, cruciform DNA structures, Holliday junctions (four-way DNA junctions), heteroduplex DNA, and also cleaves nicked double-stranded DNA at a slower rate. Cleavage occurs at the first, second, or third phosphodiester bond on the 5′ side of the mismatched base.
Features
- High Cleavage Activity: 20 U of T7 Endonuclease I efficiently cuts 200 ng of mismatched dsDNA.
- High Protein Purity: ≥95% (as determined by SDS-PAGE).
Specifications
| Source | Recombinant expression in Escherichia coli of gene 3 from bacteriophage T7 |
| Purity | ≥95% |
| Concentration | 10 U/μL |
| Cat.No. | 14548ES72 / 14548ES86 |
| Size | 250 U / 2500 U |
Components
- Components No.
- Name
- 14548ES72
- 14548ES86
- 14548-A
- T7 Endonuclease I (10 U/μL)
- 25 μL
- 250 μL
- 14548-B
- 10× T7 Endonuclease I Reaction Buffer
- 1 mL
Storage
This product should be stored at -25~-15℃ for 2 years.
Applications
- Detection of mutations following genome editing (e.g., CRISPR/Cas9 or TALEN-induced indels)
- Resolution of four-way DNA junctions or branched DNA structures
- Detection or cleavage of heteroduplex DNA and nicked DNA
- Random fragmentation of linear DNA for shotgun cloning
Figures
1. High Cleavage Activity
Figure 1. Verification of T7 Endonuclease I cleavage performance (Input: 200 ng DNA) Yeasen T7 Endonuclease I and imported Supplier A* T7 Endonuclease I were incubated with mismatched dsDNA substrates and analyzed by agarose gel electrophoresis. The results show that 20 U of Yeasen T7 Endonuclease I efficiently cleaves 200 ng of mismatched dsDNA, with a cleavage performance comparable to that of Supplier A*.
Safety Data Sheet
- 14548_MSDS_HB251209_EN.PDF
Manuals
- 14548_Manual_Ver.EN20251209.pdf
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