Product Description
The Hieff™ Stool DNA Kit is suitable for genomic DNA extraction from various types of stool samples. The silica-based material used in the centrifuge adsorption column is a proprietary new material developed by our company, which, in conjunction with our unique buffer formulation, effectively removes various inhibitory factors present in animal feces that can affect downstream experiments, and efficiently recovers genomic DNA from stool. The extracted DNA has high purity and stable quality, making it suitable for various downstream applications such as restriction digestion, PCR, library construction, and more.
Features
- 1. Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
- 2. DNA A260/A280 nm ≥1.8.
- 3. Input
- ≤
- 200 mg of stool , Stool samples including from humans, birds, rats, mice, cattle, etc.
Applications
- All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Specifications
| Cat.No. | 18820ES08 / 18820ES50 / 18820ES70 |
| Size | 5 T /50 T / 200 T |
Components
- Category
- Components No.
- Name
- 18820ES08
- 18820ES50
- 18820ES70
- Part I
- 18820-A
- Proteinase K (20 mg/mL)
- 0.1 mL
- 1 mL
- ×
- 4
- Part II
- 18820-B
- Hieff DNA Column S2
- 5 pieces
- 50 pieces
- 200 pieces
- 18820-C
- 2 mL Collection Tube
- 5 pieces
- 50 pieces
- 200 pieces
- 18820-D
- AC Buffer
- 0.5 mL
- 5 mL
- 20 mL
- 18820-E
- Lysis buffer S2
- 7 mL
- 70 mL
- 280 mL
- 18820-F
- DS agent S2
- 0.5 mL
- 5 mL
- 20 mL
- 18820-G
- Protein removal buffer S2
- 2.5 mL
- 25 mL
- 100 mL
- 18820-H
- Binding buffer S2
- 1.1 mL
- 11 mL
- 40 mL
- 18820-I
- Wash buffer*
- 1.3 mL
- 13 mL
- 50 mL
- 18820-J
- Elution buffer
- 1 mL
- 10 mL
- 20 mL
Shipping and Storage
Part I components should be stored at 2~8 ℃ for 1 year or -25~-15 ℃ for 2 years. Part II components should be stored at room temperature, valid for 1 year.
Safety Data Sheet
- 18820ES-MSDS-HB240223.PDF
Manuals
- 18820_Manual_Ver. EN20250205_EN.pdf
Citations & References
- 1. Zhang J, Tian R, Liu J, Yuan J, Zhang S, Chi Z, Yu W, Yu Q, Wang Z, Chen S, Li M, Yang D, Hu T, Deng Q, Lu X, Yang Y, Zhou R, Zhang X, Liu W, Wang D. A two-front nutrient supply environment fuels small intestinal physiology through differential regulation of nutrient absorption and host defense. Cell. 2024 Oct 31;187(22):6251-6271.e20. doi: 10.1016/j.cell.2024.08.012. Epub 2024 Oct 19. PMID: 39427662.(IF:45)
- 2. Wen S, Zhao Y, Liu S, Chen Y, Yuan H, Xu H. Polystyrene microplastics exacerbated liver injury from cyclophosphamide in mice: Insight into gut microbiota. Sci Total Environ. 2022;840:156668. doi:10.1016/j.scitotenv.2022.156668(IF:7.963)