Recombinant DNase I Set (RNase-free, Yeast) _ 14550ES yeasen
$55.00
In stock
SKU
14550ES50
Product Description
DNase I, or Deoxyribonuclease I, is an endonuclease that can digest single-stranded or double-stranded DNA. It hydrolyzes phosphodiester bonds to produce monodeoxynucleotides and oligodeoxynucleotides containing 5'-phosphate groups and 3'-OH groups. The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca 2+ and can be activated by divalent metal ions such as Co 2+ , Mn 2+ , and Zn 2+ . In the presence of Mg 2+ , DNase I can randomly cut any site of double-stranded DNA; in the presence of Mn 2+ , DNase I can cut the DNA double strand at the same site, forming a blunt end or a sticky end with 1-2 nucleotides protruding, which can be used for the treatment of various RNA samples.
Features
- Recombinant source, yeast
- RNase-free
- High enzymatic cleavage efficiency.
- More suitable for applications sensitive to RNase.
Applications
- Removal of contaminating genomic DNA from RNA samples
- Degradation of DNA templates in transcription reactions
- Removal of gDNA before RNA extraction or reverse transcription.
- Removal of template DNA in in vitro transcription.
Specifications
- Cat.N
- o
- Size
- Price
- 145
- 50
- ES
- 5
- 0
- 50 T
- 65
- 145
- 50
- ES
- 72
- 250 T
- 325
Components
- Components No.
- Name
- 1
- 4550
- ES
- 50
- 1
- 4550
- ES
- 7
- 2
- 1
- 4550
- A
- Recombinant DNase I (RNase-free, Yeast)
- 150 μL
- 750 μL
- 1
- 4550
- B
- DNase I Reaction Buffer (10×)
- 1 mL
- 5×1 mL
Shipping and Storage
This product should be stored at -25~-15℃ for two year s
Figures
RNA extraction application verification
Figure 1 RNA extraction application verification 20 U of the enzyme preparation was used to treat 12 pre-treatment samples of mouse liver from different sources. RNA extraction and agarose gel electrophoresis analysis showed that the RNA integrity of the enzyme-treated group was good, indicating that this DNase I can effectively digest DNA without affecting the quality of RNA, and fully meets the technical requirements of RNA extraction experiments.
Safety Data Sheet
- 1
- 45
- 50
- _MSDS_HB
- 250514
- _EN.PDF
Manuals
- 145
- 50
- _Manual_Ver.E
- N
- 20250514.pdf
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