Hieff™ Pro Endo-free Plasmid Maxi Kit _ 19038ES yeasen
$85.00
In stock
SKU
19038ES02
Product Description
The Hieff TM Pro Endo-free Plasmid Maxi Kit enables rapid purification of 0.5–4 mg of high-copy plasmid DNA from 50–200 mL bacterial culture (100–200 mL LB medium; 50–100 mL 2YT medium), with recovery rates reaching 80–90%. The kit is characterized by high efficiency, speed, and ease of use. The spin column utilizes a proprietary silica-based matrix material developed in-house, combined with a unique lysis buffer formulation, to maximize recovery of high-purity DNA. The inclusion of a push filter simplifies operation. Purified DNA exhibits high purity and extremely low endotoxin levels (<0.01 EU/μg DNA), making it suitable for standard and sensitive cell transfections, as well as direct applications in restriction digestion, transformation, PCR, in vitro transcription, sequencing, and other molecular biology experiments.
Features
- High Binding Capacity
- – Silica membrane columns can bind up to 4 mg of plasmid DNA.
- High Purity & Low Endotoxin
- – Plasmid endotoxin levels <0.01 EU/μg (for mid-to-high copy plasmids), significantly lower than conventional methods (>2.5 EU/μg).
- Flexible Compatibility
- – Compatible with both high-speed angled rotors and high-throughput horizontal rotors.
- Easy Operation
- – Push-filter design eliminates the need for careful supernatant handling.
Components
- Part
- No.
- Name
- 19038ES02
- 19038ES10
- Part I
- 19038-A
- RNase A (10 mg/mL)
- 250 µL
- 1200 µL
- Part II
- 19038-B
- Hieff
- TM
- DNA Column E2
- 2
- 10
- 19038-C
- 50-mL Collection Tube
- 2
- 10
- 19038-D
- Push Filter
- 2
- 10
- 19038-E
- Buffer BS (Balance Solution)*
- 10 mL
- 50 mL
- 19038-F
- Solution SI
- 25 mL
- 120 mL
- 19038-G
- Solution SII
- 25 mL
- 120 mL
- 19038-H
- Solution SIII
- 20 mL
- 100 mL
- 19038-I
- Solution ER (Endotoxin Removal Solution)
- 8 mL
- 40 mL
- 19038-J
- Solution BD (Binding Solution)*
- 1.6 mL
- 8 mL
- 19038-K
- Wash Solution (Concentrated)*
- 12 mL
- 48 mL
- 19038-L
- Elution Buffer (EB)
- 10 mL
- 30 mL
- 19038-M
- Blue Buffer
- 200 µL
- 600 µL
Storage
Part I: 19038-A (RNase A, 10 mg/mL), store at -15°C to -25°C. Stable for 18 months. Part II: All other components, store at room temperature. Stable for 18 months
Applications
- Plasmid Extraction; Endo-free Plasmid Extraction.
Figures
1. Workflow 1 . Workflow of the single-sample extraction kit, with a total processing time of ~54 minutes, from sample to purified product. 2. Higher plasmid yield Result: The Hieff TM Pro Endo-free Plasmid Maxi Kit (19038ES) achieved 1.26× higher plasmid yield compared with Supplier A*, while maintaining comparable purity. 3. Lower Endotoxin Levels 2 . Plasmid extraction performance comparison. The 19038ES kit consistently delivered plasmid DNA with endotoxin levels below 0.01 EU/µg, significantly lower than Supplier A*.
Safety Data Sheet
- 19038_MSDS_HB250919_EN.PDF
Manuals
- 19038_Manual_Ver.EN20250919.pdf
Related Blog
- Plasmid Extraction Made Simple: From Mini to Maxi in One Solution — Pure, low-endotoxin plasmid DNA for cloning, transfection, and beyond
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