Hieff™ Endo-free Plasmid Midi Kit _ 19023ES yeasen
$65.00
In stock
SKU
19023ES02
Product Description
The Hieff™ Endo-free Plasmid Midi Kit enables rapid purification of 100–500 µg of high-purity, high-copy plasmid DNA from 30–70 mL of E. coli LB (Luria-Bertani) culture. The spin column utilizes a proprietary silica-based matrix developed by our company, combined with a unique lysis buffer formulation, to maximize recovery of high-quality plasmid DNA. A specialized endotoxin-removal process ensures extremely low endotoxin levels (<0.1 EU/µg DNA), resulting in excellent performance in cell transfection. The purified plasmid is also directly suitable for a wide range of downstream molecular biology applications, including restriction digestion, transformation, PCR, in vitro transcription, sequencing, and more.
Features
- High Stability:
- Specially designed silica membranes in spin columns ensure minimal variation between columns and excellent reproducibility.
- High Yield:
- Quickly and easily extract 100–500 µg of high-copy plasmid DNA from 30–70 mL E. coli LB culture.
- Low Endotoxin:
- Unique formulation reduces endotoxin to <0.1 EU/µg DNA, ideal for efficient cell transfection.
Components
- No.
- Name
- 19023ES02
- 19023ES20
- Part I
- 19023-A
- RNase A (10 mg/mL)
- 50 µL
- 500 µL
- Part II
- 19023-B
- Endotoxin Removal Solution ER (ER Solution E2)
- 1.8 mL
- 18 mL
- 19023-C
- RS Buffer* (RS Buffer E2*)
- 5 mL
- 50 mL
- Part III
- 19023-D
- AC Buffer (AC Buffer E2)
- 1 mL
- 10 mL
- 19023-E
- Hieff™
- DNA Column E2
- 2 columns
- 20 columns
- 19023-F
- 15 mL Collection Tube E2
- 2 tubes
- 20 tubes
- 19023-G
- Lysis Buffer LB (LB Buffer E2)
- 5 mL
- 50 mL
- 19023-H
- Binding Buffer BD (BD Buffer E2)
- 5 mL
- 50 mL
- 19023-I
- Protein Removal Buffer PL* (PL Buffer E2*)
- 4 mL
- 40 mL
- 19023-J
- Wash Buffer W*
- 2.5 mL
- 25 mL
- 19023-K
- Elution Buffer
- 2 mL
- 20 mL
Storage
Part I: Store at –25°C to –15°C Part II: Store at 2–8°C Part III: Store at room temperature Valid life: 2 years
Safety Data Sheet
- 19023_MSDS_HB251212_EN.PDF
Manuals
- 19023_Manual_Ver.EN20251212.pdf
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