UCF.ME™ Uracil DNA Glycosylase (UDG/UNG), heat-labile, low DNA, 1 U/μL _ 14466ES yeasen
$150.00
In stock
SKU
14466ES60
Product Description
UCF.ME Uracil DNA Glycosylase d erived from psychrophilic marine bacteria, it is an ultra-low residual heat-labile UDG enzyme known as UCF.ME TM . This product can prevent the residual activity of conventional UDG enzymes after inactivation from degrading dU-containing amplification products at room temperature. It also prevents background bacteria present in molecular enzymes from causing false positives in PCR results. Therefore, the introduction of UCF.ME TM ultra-low residual thermolabile UDG enzyme is crucial for accurately identifying infectious pathogens and assisting in the clinical diagnosis of infections and infectious diseases.
Features
- Ultra-low residual of UCF.ME
- TM
- —
- E. coli
- genomic DNA residue <0.1 copies/ U;
- Low nuclease residue
- —
- No residual exonuclease, nicking enzyme, or RNase;
- Strong digestion capability
- —
- 0.05 U/T can digest 10
- 5
- copies/T dU-DNA products;
- Good thermolability
- —
- Complete inactivation under any condition of 50
- ℃
- for 10 minutes, 55
- ℃
- for 5 minutes, or 95
- ℃
- for 5 minutes;
- Compatible with RT-qPCR reaction systems
- —
- No impact on the detection system even at high input levels (2U/20
- μ
- L reaction system).
Specifications
| Source | Recombinant E. coli with UDG gene from psychrophilic marine bacteria |
| Storage Buffer | 20 mM Tris-HCl, 100 mM KCl, 0.1mM EDTA,1mM DTT, 0.5% Tween-20, 0.5% NP-40, 50% Glycerol, pH8.0 ± 0.2 @ 25 ℃ |
| Unit Definition | One unit (U) is defined as the amount of enzyme that required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C Heat Inactivation 55℃ for 5 min ; 50 ℃ for 10 min |
Components
- Name
- 14466ES60
- 100 U
- 14466ES76
- 500 U
- 14466ES96
- 10,000 U
- UCF.ME
- TM
- Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/
- μ
- L
- 100
- μ
- L
- 500
- μ
- L
- 10 mL
Storage
The product should be stored at -25~ -15°C for 2 years.
Figures
Figure 1. 14466ES protein purity (SDS-PAGE) detection results. Protein Purity≥95%
Figure 2. 14466ES Nuclease residue test results. No Residual Nucleases, No Residual Exonucleases, Nicking Enzymes, or RNases.
Figure 3. 14466ES E.coli genomic DNA residue test results. E.coli genomic DNA residue< 0.1 copies/ U the residue levels were significantly lower than the conventional UDG.
Figure 4. Strong digestive capability. 0.05 U/T is sufficient to digest 10^5 copies/T of dU-DNA products. 14466ES was used to prepare qPCR mix, while the control group of qPCR mix without UDG enzyme could completely digest 10 5 copies/T dU-DNA products.
Figure 5. After 50℃, 10min; 55℃, 5min; 55℃, 10min; 95℃, 5min heat inactivation treatment, 14466ES lost digestive ability.
Figure 6. Compatible with the RT-qPCR reaction system, with no impact on the detection system even at high input levels (2U/T).
Safety Data Sheet
- EN-14466-MSDS-Ver.EN20250205.pdf
Manuals
- EN_14466_Manual_Ver.EN20240426.pdf
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