Hieff UNICON™ HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL) -14316ES yeasen
$105.00
In stock
SKU
14316ES76
Product Description
Hieff UNICON TM HotStart E-Taq DNA Polymerase, Glycerol-free is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature. This product contains no Glycerol and is specially designed for the preparation of lyophilized reagents.
Specifications
| Cat.No. | 14316ES76 / 14316ES80 / 14316ES92 / 14316ES97 / 14316ES98 |
| Size | 500 U / 1000 U / 10 KU / 50 KU / 100 KU |
Components
- Name
- 14316ES76
- 14316ES80
- 14316ES92
- Hieff UNICON
- TM
- HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)
- 100 μL
- 200 μL
- 2×1 mL
Storage
This product should be stored at 2~8℃ for 1 years.
Instructions
1.Reaction Setup
Components
- Volume (μL)
- Final Concentration
- 2×Buffer
- 25
- 1×
- Primer/Probe mix
- X
- 0.1
- μmol/L-0.5
- μmol/L
- Hieff UNICON
- TM
- HotStart E-Taq DNA Polymerase, Glycerol-free (5 U/μL)
- 1.2
- 0.12 U/μL
- DNA template
- X
- 0.1-100 ng
- ddH
- 2
- O
- up to 50
- *According to the specific experimental application, the corresponding reaction Buffer should be prepared by oneself. The amount of DNA and primer concentration in the above table are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.
- Thermal cycling protocol (2-Step cycling protocol)
- Stage
- Temperature
- Time
- Cycles
- Pre-denaturation
- 95℃
- 5 min
- 1
- Denaturation
- 95℃
- 15 sec
- 45
- Annealing/Extension
- 60℃
- 30 sec
- *The reaction temperature is adjusted according to the Tm value of the designed primers. Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.
Notes
1.This product is for scientific research purposes only. 2.Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.
Manuals
- 14316_Manual_Ver.EN20230605.pdf
MDSD
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