Hieff TM anti-Taq DNA Polymerase Antibody is a Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of DNA polymerase after binding to Taq DNA Polymerase, which can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification caused by primer dimer at low temperature. Hieff TM anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, and the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibody. The storage buffer of this product is glycerol-free and can be mixed with glycerol-free Taq DNA polymerase to prepare glycerol-free hot-start Taq DNA polymerase
Rapid enzyme activity release—Over 95% of enzyme activity can be released by heating at 95°C for 20 seconds.
No Nuclease Contamination: Free from contamination of exonucleases, endonucleases, and RNases.
Applications
The concentration of this product is 5 mg/mL.
1
μL antibody
could block the activity of 20-50 U Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase well and incubate at room temperature for 20 min prior to use. The blocking efficiency is more than 90%.
Specifications
Classification
Monoclonal
Type
Antibody
Antigen
Taq DNA Polymerase
Form
Liquid
Components
Name
14225
ES60
(
1
00 µg
)
14225
ES80
(
1
mg
)
14225
ES90
(
5
mg
)
14225
ES92
(
1
0 mg
)
14225
ES93
(
1
00 mg
)
Hieff
TM
DNA Polymerase Antibody
2
0 μL
2
00 μL
1
m
L
2×1
m
L
2
0
m
L
Storage
The product should be stored at - 25 ℃~-15℃ for 2 years. For short-term use (within 2 months), the product can be stored at 2 ~ 8 ℃.