Tth DNA Polymerase (5 U/μL) _ 14607ES yeasen
$95.00
In stock
SKU
14607ES72
Product Description
Tth DNA Polymerase is a heat-resistant DNA polymerase discovered from the thermophilic bacterium Thermus thermophilus HB8 . In the presence of Mg²⁺, this enzyme exhibits 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity, but lacks 3′-5′ exonuclease activity, making it widely applicable for PCR amplification reactions. In the presence of Mn²⁺, the enzyme demonstrates strong reverse transcriptase activity at temperatures of 55-70°C, making it suitable for one-step RT-PCR reactions.
Specifications
- Unit Definition:
- One unit (U) of activity is defined as the amount of enzyme that incorporates 10 nmol of total nucleotides into an acid-insoluble product in 30 minutes at 74°C, using activated salmon sperm DNA as the template/primer.
Components
- No.
- Name
- Size
- 14607ES72
- (250 U)
- 14607ES80
- (1250 U)
- 14607-A
- Tth DNA Polymerase (5 U/μL)
- 50 μL
- 250 μL
- 14607-B
- 5×Tth RT-PCR Buffer
- 1 mL
- 4×1.25 mL
- 14607-C
- 10×Tth PCR Buffer (with Mg2+)
- 500 μL
- 2×1.25 mL
- 14607-D
- 50mM Mg(OAc)2
- 250 μL
- 1.25 mL
- 14607-E
- dNTP Mixture (10 mM each)
- 150 μL
- 750 μL
Shipping
and Storage with dry ice. Store at -20°C, with a shelf life of 2 years.
Precautions
1) If a small amount of precipitate appears in the buffer upon thawing, this is a normal phenomenon. Please invert and mix thoroughly before use. 2) For your safety and health, wear a lab coat and disposable gloves during operation. 3) This product is for research use only!
Instructions
Recommended PCR Reaction Procedure: 1. Preparation of the Reaction System Name Volume ( μL ) 10×Tth PCR Buffer (with Mg2+) 5 Tth DNA Polymerase (5 U/μL) 0.5 dNTP Mixture (10 mM each) 1 Upstream Primer 10μM 2 Downstream Primer 10μM 2 Template DNA 50 pg- 1μg ddH 2 O to 50 [Note]: The amounts of Tth DNA Polymerase, upstream and downstream primers, and dNTP Mixture can be adjusted according to specific experimental needs. 2. Cycle Setup Steps Temp( ºC ) Time Cycles Pre-denaturation 94 30 sec-5 min 1 Denaturation 94 30 sec 35 Annealing 50-70 30 sec Extension 72 60 sec/kb Final Extension 72 10 min 1 [Note]: Reaction conditions can be adjusted according to specific experimental requirements. Recommended RT-PCR Reaction Procedure: 1. Preparation of the Reaction System Name Volume ( μL ) 5×Tth RT-PCR Buffer 10 Tth DNA Polymerase (5 U/μL) 1.5 50mM Mg(OAc)2 2.5 dNTP Mixture (10 mM each) 1.5 Upstream
Primer 10μM 2 Downstream Primer 10μM 2 Template RNA 50 pg- 1μg ddH2O to 50 [Note]: The amounts of Tth DNA Polymerase, upstream and downstream primers, and dNTP Mixture can be adjusted according to specific experimental needs. 2. Cycle Setup Steps Temp ( ºC ) Time Cycles Reverse transcription 55-70 30 min 1 Pre- denaturation 94 30 sec-5 min 1 Denaturation 94 30 sec 35 Annealing 50-70 30 sec Extension 72 60 sec/kb Final Extension 72 10 min 1 [Note]: Reaction conditions can be adjusted according to specific experimental requirements.
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