Hieff™ Bst Plus DNA Polymerase (40 U/μL) _ 14402ES yeasen
$95.00
In stock
SKU
14402ES92
Product Description
Hieff™ Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I, lacking 5'-3' exonuclease activity. The enzyme has stronger 5'-3' DNA polymerase activity, strand displacement activity, and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.
Features
- Thermophilic DNA polymerase with strong strand displacement activity
Applications
- Isothermal DNA amplification
Specifications
- Polymerase
- Bst DNA Polymerase
- Heat Inactivation
- Incubation at 85°C for 5 min.
- Solution Components
- 10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH 7.5 @ 25℃.
- Activity Definition
- 1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C.
Components
- Components No.
- Name
- 14402ES92 (8,000 U)
- 14402ES97 (40,000 U)
- 14402-A
- Hieff™ Bst Plus DNA Polymerase (40 U/μL)
- 200 μL
- 1 mL
- 14402-B
- 10 ×Hieff™ Bst Plus DNA Polymerase Buffer
- 1 mL
- 3×1 mL
- 14402-C
- 100 mmol/L MgSO4
- 1 mL
- 3×1 mL
Figures
High Sensitivity
Figure 1: Sensitivity Test Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%. Stability
Figure 2.Stability testing at the same temperature Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days 0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test Red: Repeat freeze-thaw 0 times Blue: Repeat freeze-thaw 5 times Green: Repeat freeze-thaw 10 times Specificity
Figure 4. 48-well NTC-specific assay 48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.
Storage
The product should be stored at -25℃ ~ -15℃ for 2 years. Please avoid repeated freeze-thaw.
Safety Data Sheet
- 14402_MSDS_14402_HB251125.pdf
Manuals
- 14402_Manual_HB230115_EN.pdf
Related Blog
- What is the role of Bst DNA polymerase in LAMP?
- High-quality Bst Enzyme Lyophilized Material Makes Molecular POCT More Convenient
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