RT-LAMP Lyo-Ready Fluorescent Assay Kit(Bst Plus) _ 16730ES yeasen
$325.00
In stock
SKU
16730ES60
Product Description
The RT-LAMP Lyo-Ready Fluorescent Assay Kit (Bst Plus) is a kit capable of performing isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant Hifair TM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve efficient, rapid, and highly specific detection reactions. It also supports room-temperature reaction system preparation. The buffers and enzyme components in the kit do not contain glycerol and can be lyophilized using the provided lyophilization protectant.
Features
- s
- Broad Compatibility
- : Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity:
- Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity:
- Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability:
- Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
- Components No.
- Name
- 16730ES60
- 16730ES80
- 16730-A
- 4×Lyo LAMP Buffer
- 600 µL
- 6 mL
- 16730-B
- Bst Plus DNA Polymerase(Glycerol Free)
- 100 µL
- 1 mL
- 16730-C
- RT Enzyme mix(Glycerol Free)
- 50 µL
- 500 µL
- 16730-D
- 25×Fluorescent Dye
- 100 µL
- 1 mL
- 16730-E
- Lyophilization stabilizer
- 800 µL
- 8
- mL
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Applications
- Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow. 2. Consistent performance between lyophilized and liquid formats.
Figure 2. Comparable Detection Performance of Liquid and Lyophilized Formats. Both liquid and lyophilized versions of the RT-LAMP assay exhibit consistent sensitivity and specificity in detecting DNA and RNA targets, with similar amplification curves and reaction times.
Safety Data Sheet
- 16730_MSDS_HB250713_EN.PDF
Manuals
- 16730_Manual_Ver.EN20250708.pdf
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