RT-LAMP Fluorescent Assay Kit(Bst Plus) _ 16729ES yeasen
$325.00
In stock
SKU
16729ES60
Product Description
The RT-LAMP Fluorescent Assay Kit (Bst Plus) is a kit capable of isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve highly efficient, rapid, and highly specific detection reactions, and supports room-temperature reaction system preparation.
Features
- s
- Broad Compatibility
- : Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity:
- Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity:
- Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability:
- Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
- Components No.
- Name
- 16729ES60
- 16729ES80
- 16729-A
- 4×LAMP Buffer
- 600 µL
- 6 mL
- 16729-B
- Bst Plus DNA Polymerase
- 100 µL
- 1
- mL
- 16729-C
- RT Enzyme mix
- 50 µL
- 500 µL
- 16729-D
- 25×Fluorescent Dye
- 100 µL
- 1
- mL
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Applications
- Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow. 2. Broad Compatibility: DNA Pathogen Detection&RNA Pathogen Detection
Figure 2. Broad Pathogen Detection Compatibility. (A) DNA pathogen detection: Reliable amplification observed across multiple DNA virus targets. (B) RNA pathogen detection: Effective detection of RNA viruses with high sensitivity and specificity. 3. High Specificity
Figure 3. High Specificity of RT-LAMP Assay (Cat#16729) Compared to Supplier A* Specificity was evaluated using both DNA and RNA templates, including positive targets and non-target (negative) controls. The RT-LAMP assay (Cat#16729) showed no false positives and clear separation between positive and negative samples, outperforming Supplier A* by avoiding non-specific amplification. 4. Strong stability
Figure 4. Stability Evaluation of the RT-LAMP System. (A) Freeze-thaw stability: Performance remains consistent after 20 freeze-thaw cycles. (B) Thermal accelerated stability: Maintains amp lification efficiency after 7 days of storage at 37°C.
Safety Data Sheet
- 16729_MSDS_HB250713_EN.PDF
Manuals
- 16729_Manual_Ver.EN20250708.pdf
Reviews
Write Your Own Review