RT-LAMP Dye Assay Kit (UDG plus) Fluorescent-13762ES yeasen
$320.00
In stock
SKU
13762ES60
Product Description
RT-LAMP Dye Assay Kit (UDG plus) contains fluorescent dye, Hieff™ Bst Plus DNA Polymerase, heat-resistant Hifair™ Ⅲ Reverse Transcriptase, MRI, and UDGas applied to LAMP reaction with RNA as template. This product is with high sensitivity and high specificity.
Features
- High Sensitivity：50 copies/T, 25 μL system;
- High stability: stable at 37°C for 5 days with good stability, and can withstand repeated freezing and thawing more than 10 times;
- Specificity: 48-well NTC has no peaks at all, and the specificity is strong;
Applications
- Rapid detection of pathogenic bacteria
- Rapid detection of pet pathogens
- On-site detection of animal husbandry
Specifications
| Detection Method | Fluorescent dye method PCR method RT-LAMP Polymerase Hieff™ Bst Plus DNA Polymerase Type of sample RNA LOD 50 copies/T |
| Specificity | 100% |
Components
- Components No.
- Name
- 13762ES60 (100 T)
- 13762ES80 (1000 T)
- 13762-A
- Bst Buffer
- 600 µL
- 6 mL
- 13762-B
- Bst Enzyme Mix（UDG plus）
- 100 µL
- 1 mL
Storage
Part 1: 13762-A should be protected from light and can be stored at -20°C for 6 months. Part 2: 13762-B can be stored at -20°C for 6 months.
Figures
High Sensitivity
Figure 1: Sensitivity Test Red: 50 copies/T, response less than 15 minutes
Figure 1: The copy numbers of 50 copies/Tweres tested. The test results showed that within 15 minutes, the results of 50 copies/T performed better, and the detection rate reached 100%. Stability
Figure 2.Stability testing at the same temperature Red: 37℃ 0 day Blue: 37℃ 1 day Green: 37℃ 3 days Purple: 37℃ 5 days 0 days at 37°C as a control and stability tests were carried out for 1, 3, and 5 days at the same temperature, indicating that it can still maintain a relatively stable effect at 37°C for 5 days.
Figure 3. -20℃ repeated freeze-thaw stability test Red: Repeat freeze-thaw 0 times Blue: Repeat freeze-thaw 5 times Green: Repeat freeze-thaw 10 times Specificity
Figure 4. 48-well NTC-specific assay 48-well NTC detection was used in the specific detection of the product, and no peak was detected, indicating that the specificity was better.
Documents
- MSDS
- EN-13762-A-MSDS-HB220728.pdf
- EN-13762-B-MSDS-HB220728.pdf
Manuals
- 13762_Manual_HB220708_EN.pdf
Related Blog
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