Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES yeasen
$90.00
In stock
SKU
20543ES10
Product Description
Protein A is a cell wall component produced by several strains of Staphylococcus aureus, consisting of a polypeptide chain and containing almost no carbohydrates. This product is produced in Escherichia coli and functions similarly to natural protein A. It consists of five IgG binding domains E-D-A-B-C arranged in series, with strong specific affinity for the Fc fragment of IgG. Protein A, as an affinity ligand, is coupled to the matrix and can specifically bind to antibody molecules in the sample, allowing other impurities to flow through and exhibiting high selectivity. Commonly used for purification of monoclonal antibodies or polyclonal antibodies. Protein A binds to human, mouse, and rat IgG subclasses (e.g. human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c), as well as total IgG from rabbits, guinea pigs, pigs, dogs, and cats.
Components
- No.
- Name
- 20543ES10
- 20543ES60
- 20543
- Recombinant Alkalitolerant Protein A
- 10
- mg
- 100 mg
- Product
- Properties
- Synonyms
- Recombinant
- protein A alkali-tolerant
- Source
- E.coli
- Molecular
- Weight
- Approximately
- 26.7
- kDa, a sing
- le non-glycosylated
- polypeptide chain
- containing
- 237amino acids
- Amino
- Acid
- Sequence
- AQGTVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEA
- KKLND
- AQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAE
- AKKLN
- DAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDP
- SQSANLLAEAKKL
- NDAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKD
- DPSQSANLLAEAKK
- LNDAQAPKC
- Concentration
- for
- 1
- unity
- OD
- at
- 280
- nm
- 4.4
- mg/mL
- Physical
- Appearance
- > 97
- %
- by
- SDS-PAGE and
- HPLC analyses.
- Purity
- > 97%
- by SDS-PAGE and
- HPLC
- analyses.
- Endotoxin
- Level
- <0.1
- EU /1 μg of the
- protein
- by the
- LAL
- method.
- Formulation
- A 0.2 µm filtered solu
- tion in 20
- mM
- PB,400 mM
- NaCl,
- pH7.4 or
- lyophilized from
- a
- 0.2 µm filtered solution in
- 20
- mM
- PB,400
- mM
- NaCl,
- pH7.4
- Reconstitution
- Dissolve in distilled water or saline.
Storage
This product should be stored at -25~ -15℃, valid for 12 months. Avoid repeated freeze-thaw cycles.
Notes
1. Please operate with lab coats and disposable gloves for your s afety. 2. This product is for research use only. Appendix Table 1. Summary of Binding Abilities of Proteins A, G, and L to Ig from Different Species
Safety Data Sheet
- 20543-MSDS-HB250701
Manuals
- 20543-Manual-Ver.EN20250701
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