Product Description
Product Properties Alternative Names PDCD1, PD1, CD279, SLEB2, PD-1 Species Canine Expression Sequence Leu25-Val170 Accession NP_001301026.1 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 43.00 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Canine PD-1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, mFc Tag with the EC50 of 30.7ng/ml determined by ELISA . Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.
Features
Storage
Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.