Product Description
Product Properties Alternative Names IL-22R-alpha-1, IL-22RA1, CRF2-9, IL22R, IL22R1, IL-TIF-R1, zcytoR11 Species Rat Expression Sequence His16-Ala228 Accession NP_001178798.1 Tag C-hFc Expression System HEK293 Molecular Weight The protein has a predicted MW of 51.08 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS, 100m L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Human IL-22, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Rat IL-22R alpha 1, hFc Tag with the EC50 of 30.4ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.
Features
Storage
Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.