Product Description
Product Properties Alternative Names Fibroleukin, pT49, FGL2, fibrinogen-like 2, Species Rat Expression Sequence Val193-Pro427 Accession Q32Q89 Tag N-His-Avi,N-Flag Expression System HEK293 Molecular Weight The protein has a predicted MW of 31.31 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Rat FGL2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 0.29μg/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.
Features
Storage
Store at -85 to -65°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.