Product Description
Product Properties Alternative Names Fibroleukin, pT49, FGL2, fibrinogen-like 2, Species Mouse Expression Sequence Pro197-Pro432 Accession P12804 Tag N-His-Avi,N-Flag Expression System HEK293 Molecular Weight The protein has a predicted MW of 31.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE and HPLC. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Biotinylated Mouse FGL2, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. Reconstitution Method Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles.
Features
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.