Product Description
This product is a linearized polyethyleneimine (Polyethylenimine Linear, PEI) specially developed for the large-scale production of AAV in suspension culture systems. It is a chemically synthesized product, free of animal-derived components, with low cytotoxicity. The product is provided in sterile liquid form and has a wide applicability for various AAV serotypes. It features strong DNA loading capacity, low transfection complex ratio, and high complex stability, facilitating large-scale AAV and protein production.
Features
Applications
Specifications
Components
Shipping and Storage
Hieff Trans™ UltraAAV Transfection Reagent products should be stored at 2-8 ℃ for 2 year.
Figures
1. Cost-Efficient & Minimal Reagent Use
Figure 1. Hieff Trans™ UltraAAV achieves more with less—minimizing reagent input while maximizing protein production efficiency. 2. High-efficiency transfection
Figure 2. Comparison of transfection performance between Yeasen Hieff TransTM (PEI AAV) and Control PEI(FectorVIR-AAV). Hieff TransTM requires only half the reagent input yet still achieves higher viral yield than Control PEI. At equivalent viral production, the cost of transfection reagent is reduced by 50%. 3. Maximized AAV Yield
Figure 3. Hieff Trans™ UltraAAV maximizes AAV yield across multiple serotypes. Superior transfection efficiency was observed compared with leading PEIs. AAV2, AAV5, AAV8, and AAV9 were produced in suspension 293F cells using 1 µg DNA per million cells. Viral particles were harvested 72 hours post-transfection, and supernatants were analyzed. 4. Strong Stability
Figure 4. Stability evaluation in the AAV9 project. Hieff TransTM demonstrated consistent performance across three GMP-grade batches and passed both accelerated and transport stability testing, ensuring reliable and stable supply. Related Reading Revolutionary PEI-Mediated Gene Delivery for Superior Transfection Efficiency
Safety Data Sheet
Manuals