Product Description
Product Properties Alternative Names AGE-R3, CBP35, GAL3, Gal-3, galactin-3, GALBPCBP35, Galectin3, GALIG, L29, L31, Lectin L-29, LGALS2, LGALS3, Mac-2, MAC2GAL3 Species Cynomolgus Expression Sequence Ala2-Ile248 Accession XP_005561371.1 Tag N-His Expression System HEK293 Molecular Weight The protein has a predicted MW of 27.05 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on SDS-PAGE result. Purity > 95% as determined by SDS-PAGE. Endotoxin < 1.0 EU per μg by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in 50mM Hepes, 150mM NaCl, 200mM L-arginine, 2mM Tcep (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization. Appearance Lyophilized Powder Biological Activity 【ELISA Data】 Immobilized Cynomolgus Galectin 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 38.4ng/ml determined by ELISA.
Reconstitution Method Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM Hepes, 150mM NaCl, 200mM L-arginine, 2mM Tcep (pH 6.5).
Features
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.