Product Description
Endo S, is a highly specific glycosidase derived from Streptococcus pyogenes. It can cut the N-linked glycans between the core structure of the shell di-saccharides of wild-type IgG heavy chains. This product has a purity of over 95%, high activity, good stability, no glycosidase contamination, and no proteolytic activity. For easy subsequent operations, it is equipped with a histidine (His) tag, which makes it easy to remove from the reaction. Properties English synonym Endo S Source E.coli recombinant expression Purity Detected by SDS-PAGE, purity > 95% Storage buffer PBS pH 7.5 Specific activity 8,000 U/mg Unit Definition The definition of a unit is that under a total reaction volume of 10 μL, at 37 °C for 1 hour, 95% of the N-linked glycans in 5 μg of natural mouse IgG can be removed.
Components
- Components No.
- Name
- 20413ES80
- 20413ES90
- 20413-A
- Endo S
- 1000 U
- 5×1000 U
- 20413-B
- 10×Buffer
- 1 mL
- 5×1 mL
Storage
The product can be stored at -15~-25℃ for one year.
Instructions
Reaction systerm
Components
- Volume
- IgG
- V
- Endo S
- 1
- 10×Buffer
- 1
- H
- 2
- O
- to 10
- Note: After incubation at 37 °C for 1 hour, electrophoresis detection, load 2-3 μg of samples. The control group is the original protein.
- Experimental Example
- Electrophoresis of IgG under Endo S enzyme conditions
- Lane -: Control, without Endo S enzyme; Lane +: With Endo S enzyme.
Notes
1. Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety ！ 2. For research use only! Ver. EN20240426
Manuals
- 20413_Manual_HB240528_EN.pdf
MDSD
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
