3C Protease, Expressed in E.coli. _ 20409ES yeasen
$105.00
In stock
SKU
20409ES60
Product Description
Lysine endopeptidase (Lys-C) is a serine protease derived from Pseudomonas species, which recognizes lysine in proteins and can specifically hydrolyze amide, ester, and peptide bonds on the carboxyl side of lysine. This product is a recombinant lysine endopeptidase expressed by Escherichia coli, free from other miscellaneous proteases (such as Lys-N, Arg-C, and other serine proteases), and has the same specificity and activity as natural lysine endopeptidase. Additionally, our company also provides recombinant trypsin (mass spectrometry grade) (Cat#20416ES), which cuts at the carboxyl end of lysine or arginine residues in proteins. Customers can choose according to their experimental needs.
Features
- 1.High activity
- good stability
- 2.High purity of recombinant protein, free from other miscellaneous proteases, and no animal origin.
Applications
- 1.Protein analysis, such as protein mass spectrometry, sequencing, and peptide mapping analysis.
- 2.Synthesis of lysine-related derivatives.
Specifications
| Source | E.Coli recombinant expression |
| Molecular Weight | 29±3 kDa |
| Purity | ＞ 95% |
| Specific Activity | ≥3.0 AU/mg SDS-PAGE Single main band |
| Concentration | 1.0 mg/mL |
| Storage Buffer | 5 mM NaAc 10 mM EDTA pH=5.5 |
| Unit Definition | Under the condition of 25 mmol/L pH9.0 Tris-HCl buffer, at 37℃, the amount of enzyme required to catalyze the production of 1 μmol AC-Lys-pNA in 1 min is defined as one activity unit (AU) |
Storage
The product can be stored at -25 ~ -15 ℃ for one years.
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