Product Description
Product Properties Property Name Property Details Alternative Names C1 esterase, Complement C1s subcomponent Species Human Expression Sequence Glu16-Asp688 Accession P09871 Tag C-His Expression System HEK293 Molecular Weight Predicted MW: 75.98 kDa. Due to enzyme lysis glycosylation, migrates to 55-60 kDa (light chain) 32-35 kDa (heavy chain) , and 76-96 kDa (SDS-PAGE) Purity >95% (SDS-PAGE) Endotoxin <1.0 EU/μg (LAL method) Formulation Lyophilized from 0.22 μm filtered PBS (pH 7.4) + 8% trehalose Appearance Lyophilized Powder Biological Activity 【ELISA Data】Immobilized Human Complement Component C1S, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-C1S Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. Reconstitution Method 1. Centrifuge before opening 2. Reconstitute with distilled water (>100 μg/mL) 3. Aliquot to avoid freeze-thaw cycles
Features
Storage
Lyophilized powder : Store at -25 to -15°C ; stable for 1 year upon receipt. Reconstituted protein : Store under sterile conditions at -85 to -65°C ; stable for 3 months