Human CD8 alpha&beta Heterodimer Protein, His-Flag Tag yeasen
$185.00
In stock
SKU
95092ES25
Product Description
Product Properties Property Name Property Details Alternative Names CD8A&CD8B (CD8 αβ heterodimer) Species Human Expression Sequence Ser22-Asp182 (CD8 α acidic tail) & Leu22-Pro170 (CD8 β basic tail) Accession P01732-1 (CD8 α) & P10966-1 (CD8 β) Tag C-His-Flag Expression System HEK293 Molecular Weight Predicted MW: 23.54 kDa (CD8 α), 22.62 kDa (CD8 β). Glycosylation shifts migration to 26-28 kDa (CD8 α) and 28-32 kDa (CD8 β) (SDS-PAGE). Purity >95% (SDS-PAGE & HPLC). Endotoxin <1.0 EU/μg (LAL method). Formulation Lyophilized from 0.22 μm filtered PBS (pH 7.4). 8% trehalose added as protectant. Appearance Lyophilized Powder Biological Activity 【 ELISA Data 】 Immobilized Human CD8A&CD8B, His Tag at 0.5 μg/mL (100 μL/well). Dose response curve for Anti-CD8 Antibody (hFc Tag) shows EC₅₀ = 15.2 ng/mL. Reconstitution Method Centrifuge before opening. Reconstitute with distilled water to >100 μg/mL. Aliquot to avoid repeated freeze-thaw cycles.
Features
- High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Lyophilized powder : Store at -25 to -15°C; stable for 1 year upon receipt. Reconstituted protein : Store under sterile conditions at -85 to -65°C; stable for 3 months.
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