Lambda Exonuclease L747582-1KU Aladdin
$199.00
In stock
SKU
L747582-1KU
Product Information
| Product Name | Lambda Exonuclease |
| Accession | P03697.1 |
| Specifications & Purity | Animal Free, Carrier Free, ActiBioPure™, Bioactive, EnzymoPure™, sterile, RNase free, ≥95%(SDS-PAGE), 5.0 U/μL |
| Biological Activity | Lambda exonuclease is a highly processive 5'→3' exodeoxyribonuclease, selectively digests the 5’-phosphorylated strand of double-stranded DNA, exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has limited activity at gaps in DNA. Activity Definition: One unit is defined as the amount of enzyme required to produce 10nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in atotal reaction volume of 50 μ l in 30minutes at 37 ℃ in 1X LambdaExonuclease Reaction Buffer with 1μg sonicated duplex [3H]-DNA. |
| Synonyms | λ Exonuclease | exo | lambdap24 |
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | Store at -20℃ long term (24 months). Avoid freeze/thaw cycle. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Additional Information
| Unit Definition | One unit is defined as the amount of enzyme required to produce 10nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50μl in 30minutes at 37℃ in 1X Lambda Exonuclease Reaction Buffer with 1μg sonicated du |
| Concentration | 5.0 U/μL |
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