Recombinant 3-Hydroxybutyrate Dehydrogenase (HBDH), CAS No.9028-38-0 D1493002-100U Aladdin
$19.00
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SKU
D1493002-100U
Product Information
| Product Name | Recombinant 3-Hydroxybutyrate Dehydrogenase (HBDH), CAS No.9028-38-0 |
| CAS | 9028-38-0 |
| Specifications & Purity | Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),≥300U/mg enzyme powder; ≥500U/mg protein |
| Biological Activity | ≥300U/mg enzyme powder; ≥500U/mg protein |
| Synonyms | β-Hydroxybutyrate Dehydrogenase | (R)-3-Hydroxybutanoate:NAD+ oxidoreductase | 3-HBDH | D-3-Hydroxybutyrate dehydrogenase | D-beta-hydroxybutyrate dehydrogenase | 3-D-hydroxybutyrate dehydrogenase | bdhA |
Product Description
3-Hydroxybutyrate Dehydrogenase (3-HBDH) is a mitochondrial enzyme, is often used in biochemical studies. 3-hydroxybutyrate dehydrogenase is involved in the synthesis and degradation of ketone bodies and butanoate metabolism. 3-Hydroxybutyrate dehydrogenase catalyzes (R)-3-hydroxybutanoate converts into acetoacetate.
Reaction
Product description
Appearance:
White amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 1.1.1.30
CAS Number:
9028-38-0
Storage temperature:
-20℃
Activity:
≥300U/mg enzyme powder; ≥500U/mg protein
Unit definition:
One unit converts one micromole of 3-hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.
Properties
Stability:
Stable at -20℃ for at least five years
Molecular weight:
27kDa (SDS-PAGE)
Isoelectric point:
7.2
Michaelis constant:
2.1×10⁻³M (D-3-Hydroxybutyrate)
Optimum pH:
7.0~9.0
{Fig. 1}
Optimum temperature:
60℃
{Fig. 3}
pH Stability:
6.0~11.0 (25℃, 24hr)
{Fig. 2}
Thermal stability:
< 37℃ (pH 8.5, 30min)
{Fig. 4}
Inhibitors:
Cu²⁺, Fe³⁺, Zn²⁺, NEM, Proclin, SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on HBDH
[The enzyme dissolved in 50mM Tris-HCl buffer, pH 8.5 (20U/ml) was incubated with each chemical at 37℃ for 2hr.]
Reconstitution
Reconstitute in water.
Storage and Shipping
| Shipped In | Ice chest + Ice pads |
| Stability and Storage | Store at -20℃ long term (5 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated. |
| Storage Temp | Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing |
Additional Information
| EC Number | 232-830-6 |
| Unit Definition | One unit converts one micromole of 3-Hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃. |
| Concentration | ≥300U/mg enzyme powder; ≥500U/mg protein |
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