Human Ghrelin(GHRL) ELISA Kit CSB-E13398h CUSABIO
$50.00
In stock
SKU
CSB-E13398h
Product Details
| Target Name | ghrelin/obestatin prepropeptide |
| Uniprot No. | Q9UBU3 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.156 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | Appetite regulating hormone ELISA Kit; Ghrelin 27 ELISA Kit; Ghrelin 28 ELISA Kit; Ghrelin and obestatin prepropeptide ELISA Kit; Ghrelin ELISA Kit; Ghrelin/obestatin prepropeptide ELISA Kit; ghrl ELISA Kit; GHRL_HUMAN ELISA Kit; Growth hormone releasing peptide ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; M46 protein ELISA Kit; Motilin related peptide ELISA Kit; Motilin-related peptide ELISA Kit; MTLRP ELISA Kit; Obestatin ELISA Kit; Protein M46 ELISA Kit |
| Abbreviation | GHRL |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range | 0.625 pg/mL-40 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cardiovascular |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 86; 1: Range %; 2: 80-92; 1: 1:200; 2: Average %; 3: 99; 1: Range %; 2: 91-105; 1: 1:400; 2: Average %; 3: 101; 1: Range %; 2: 92-110; 1: 1:800; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-98; 1: EDTA plasma (n=4); 2: 94; 3: 90-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 40 2.921 2.903 2.912 2.826 20 2.405 2.348 2.383 2.297 10 1.663 1.643 1.653 1.567 5 1.053 0.989 1.021 0.935 2.5 0.575 0.603 0.589 0.503 1.25 0.322 0.319 0.321 0.235 0.625 0.214 0.229 0.222 0.136 0 0.089 0.083 0.086; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 40; 9: 2.921; 10: 2.903; 11: 2.912; 12: 2.826; 14: 20; 15: 2.405; 16: 2.348; 17: 2.383; 18: 2.297; 20: 10; 21: 1.663; 22: 1.643; 23: 1.653; 24: 1.567; 26: 5; 27: 1.053; 28: 0.989; 29: 1.021; 30: 0.935; 32: 2.5; 33: 0.575; 34: 0.603; 35: 0.589; 36: 0.503; 38: 1.25; 39: 0.322; 40: 0.319; 41: 0.321; 42: 0.235; 44: 0.625; 45: 0.214; 46: 0.229; 47: 0.222; 48: 0.136; 50: 0; 51: 0.089; 52: 0.083; 53: 0.086; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 40; 2: 2.921; 3: 2.903; 4: 2.912; 5: 2.826; 1: 20; 2: 2.405; 3: 2.348; 4: 2.383; 5: 2.297; 1: 10; 2: 1.663; 3: 1.643; 4: 1.653; 5: 1.567; 1: 5; 2: 1.053; 3: 0.989; 4: 1.021; 5: 0.935; 1: 2.5; 2: 0.575; 3: 0.603; 4: 0.589; 5: 0.503; 1: 1.25; 2: 0.322; 3: 0.319; 4: 0.321; 5: 0.235; 1: 0.625; 2: 0.214; 3: 0.229; 4: 0.222; 5: 0.136; 1: 0; 2: 0.089; 3: 0.083; 4: 0.086 |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GHRL antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled GHRL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Ghrelin is a peptide hormone primarily produced by ghrelinergic cells in the gastrointestinal tract, particularly the stomach fundus. This 28-amino acid hormone regulates appetite and energy homeostasis by stimulating growth hormone release and promoting food intake. Ghrelin levels typically rise before meals and fall after eating, making it a key regulator of hunger and satiety. The hormone also influences gastric motility, glucose metabolism, and cardiovascular function. These properties make ghrelin important in metabolic research and studies of eating disorders, obesity, and metabolic syndrome. The Human Ghrelin ELISA Kit (CSB-E13398h) provides quantitative measurement of ghrelin/obestatin prepropeptide in human samples. This sandwich-based assay works with serum, plasma, cell culture supernatants, and tissue homogenates. The detection range spans 0.625-40 pg/mL with sensitivity of 0.156 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in clinical research studies to measure ghrelin levels in human serum and plasma samples. The applications span metabolic and endocrine research contexts where ghrelin quantification serves as a biomarker for various physiological conditions. • Metabolic research: Measurement of fasting serum ghrelin concentrations in studies examining metabolic parameters and hormonal profiles • Endocrine profiling: Quantification of both acyl and desacyl ghrelin forms in plasma samples as part of comprehensive hormone analysis • Clinical biomarker studies: Analysis of ghrelin levels in peripheral blood samples from human subjects in controlled research settings • Comparative studies: Analysis of ghrelin concentrations alongside other metabolic hormones to study hormonal interactions and regulatory pathways |
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