Human Interferon γ (IFN-γ) ELISA Kit CSB-E04577h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04577h-IS
Product Details
| Uniprot No. | P01579 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.95 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | IF 1 ELISA Kit; IFG ELISA Kit; IFI ELISA Kit; IFN gamma ELISA Kit; IFN immune ELISA Kit; IFN, immune ELISA Kit; IFN-gamma ELISA Kit; IFNG ELISA Kit; IFNG_HUMAN ELISA Kit; Immune interferon ELISA Kit; Interferon gamma ELISA Kit; Type II Interferon ELISA Kit |
| Sample Types | serum, plasma, tissue homogenates, cell culture supernates |
| Detection Range | 7.8 pg/ml-500 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 92; 1: Range %; 2: 87-96; 1: 1:2; 2: Average %; 3: 95; 1: Range %; 2: 92-99; 1: 1:4; 2: Average %; 3: 91; 1: Range %; 2: 87-94; 1: 1:8; 2: Average %; 3: 89; 1: Range %; 2: 86-93; |
| Recovery | headers: The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 91; 3: 88-96; 1: EDTA plasma (n=4); 2: 95; 3: 89-99; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; ; ; ; ; ; ; ; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 500; 2: 2.046; 3: 2.058; 4: 2.052; 5: 1.902; 1: 250; 2: 1.537; 3: 1.444; 4: 1.491; 5: 1.341; 1: 125; 2: 1.027; 3: 0.987; 4: 1.007; 5: 0.857; 1: 62.5; 2: 0.648; 3: 0.635; 4: 0.642; 5: 0.492; 1: 31.2; 2: 0.456; 3: 0.432; 4: 0.444; 5: 0.294; 1: 15.6; 2: 0.342; 3: 0.345; 4: 0.344; 5: 0.194; 1: 7.8; 2: 0.241; 3: 0.254; 4: 0.248; 5: 0.098; 1: 0; 2: 0.151; 3: 0.149; 4: 0.150; |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of human interferon-gamma (IFN-γ), a pivotal type II interferon produced primarily by T cells and NK cells that orchestrates cell-mediated immune responses. Because IFN-γ levels can span from near-undetectable baseline concentrations in healthy plasma to sharply elevated values during active immune activation, the kit is engineered to capture this dynamic range across diverse experimental contexts. Detection Range: The 7.8–500 pg/ml range aligns with the physiological and pathological concentrations of circulating IFN-γ, from low resting levels in healthy serum to elevated concentrations seen during infection, graft rejection, or Th1-driven inflammation. Mitogen-stimulated PBMC supernatants may require dilution to fall within range, while unstimulated samples can typically be assayed neat. Sensitivity: At 1.95 pg/ml, the kit detects the low basal IFN-γ levels present in unstimulated plasma or early-phase immune responses, where concentrations often fall below 10 pg/ml. Sample Compatibility: Validation across serum, plasma, tissue homogenates, and cell culture supernatants enables researchers to correlate systemic IFN-γ levels with local tissue production or ex vivo T cell and NK cell functional assays within a single study. Assay Efficiency: The 1–5 hour protocol and 50–100 µl sample volume accommodate limited-volume specimens from PBMC cultures or pediatric draws while supporting high-throughput screening of stimulation conditions. This kit is directly applicable to tuberculosis immune monitoring, transplant rejection surveillance, and Th1/Th2 polarization studies. |
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