Human Interferon β,IFN-β/IFNB ELISA Kit CSB-E09889h CUSABIO
$50.00
In stock
SKU
CSB-E09889h
Product Details
| Target Name | interferon, beta 1,fibroblast |
| Uniprot No. | P01574 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 7.8 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | beta-interferon ELISA Kit; Fibroblast interferon ELISA Kit; IFB ELISA Kit; IFF ELISA Kit; IFN beta ELISA Kit; IFN-beta ELISA Kit; IFNB 1 ELISA Kit; IFNB ELISA Kit; IFNB_HUMAN ELISA Kit; IFNB1 ELISA Kit; Interferon beta 1 fibroblast ELISA Kit; Interferon beta ELISA Kit; Interferon beta precursor ELISA Kit; MGC96956 ELISA Kit |
| Abbreviation | IFNB1 |
| Sample Types | serum, urine, cell culture supernates, tissue homogenates, cerebrospinal fluid (CSF) |
| Detection Range | 31.25 pg/mL-2000 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 84; 1: Range %; 2: 80-89; 1: 1:2; 2: Average %; 3: 100; 1: Range %; 2: 91-110; 1: 1:4; 2: Average %; 3: 97; 1: Range %; 2: 92-105; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 89-98 |
| Recovery | headers: The recovery of human IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 89-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 2000 2.523 2.416 2.470 2.356 1000 2.214 2.128 2.171 2.057 500 1.803 1.724 1.764 1.650 250 1.261 1.225 1.243 1.129 125 0.765 0.716 0.741 0.627 62.5 0.463 0.445 0.454 0.340 31.25 0.289 0.274 0.282 0.168 0 0.115 0.113 0.114; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 2000; 9: 2.523; 10: 2.416; 11: 2.470; 12: 2.356; 14: 1000; 15: 2.214; 16: 2.128; 17: 2.171; 18: 2.057; 20: 500; 21: 1.803; 22: 1.724; 23: 1.764; 24: 1.650; 26: 250; 27: 1.261; 28: 1.225; 29: 1.243; 30: 1.129; 32: 125; 33: 0.765; 34: 0.716; 35: 0.741; 36: 0.627; 38: 62.5; 39: 0.463; 40: 0.445; 41: 0.454; 42: 0.340; 44: 31.25; 45: 0.289; 46: 0.274; 47: 0.282; 48: 0.168; 50: 0; 51: 0.115; 52: 0.113; 53: 0.114; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 2000; 2: 2.523; 3: 2.416; 4: 2.470; 5: 2.356; 1: 1000; 2: 2.214; 3: 2.128; 4: 2.171; 5: 2.057; 1: 500; 2: 1.803; 3: 1.724; 4: 1.764; 5: 1.650; 1: 250; 2: 1.261; 3: 1.225; 4: 1.243; 5: 1.129; 1: 125; 2: 0.765; 3: 0.716; 4: 0.741; 5: 0.627; 1: 62.5; 2: 0.463; 3: 0.445; 4: 0.454; 5: 0.340; 1: 31.25; 2: 0.289; 3: 0.274; 4: 0.282; 5: 0.168; 1: 0; 2: 0.115; 3: 0.113; 4: 0.114 |
| Materials provided | A micro ELISA plate --- The 96-well plate has been pre-coated with anti-human IFN-β This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-labeled IFN-βantibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody. HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution. HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution. Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration. Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances. TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator that can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human IFNB1 ELISA Kit was designed for the quantitative measurement of Human IFNB1 protein in serum, urine, cell culture supernates, tissue homogenates, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL. |
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