Human Macrophage Inflammatory Protein-1α,MIP-1α ELISA kit CSB-E04662h-IS CUSABIO
$50.00
In stock
SKU
CSB-E04662h-IS
Product Details
| Uniprot No. | P10147 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 1.17 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | C C motif chemokine 3 ELISA Kit; CCL 3 ELISA Kit; CCL3 ELISA Kit; CCL3_HUMAN ELISA Kit; Chemokine (C C motif) ligand 3 ELISA Kit; Chemokine C C motif ligand 3 ELISA Kit; Chemokine ligand 3 ELISA Kit; G0/G1 switch regulatory protein 19 1 ELISA Kit; G0/G1 switch regulatory protein 19-1 ELISA Kit; G0S19 1 ELISA Kit; G0S19 1 protein ELISA Kit; Heparin binding chemotaxis protein ELISA Kit; L2G25B ELISA Kit; LD78 alpha ELISA Kit; LD78-alpha(4-69) ELISA Kit; LD78alpha ELISA Kit; Macrophage inflammatory protein 1 alpha ELISA Kit; Macrophage inflammatory protein 1-alpha ELISA Kit; macrophage inflammatory protein 1a ELISA Kit; MIP 1 alpha ELISA Kit; MIP 1A ELISA Kit; MIP-1-alpha ELISA Kit; MIP-1-alpha(4-69) ELISA Kit; MIP1 alpha ELISA Kit; MIP1A ELISA Kit; PAT 464.1 ELISA Kit; SCYA 3 ELISA Kit; SCYA3 ELISA Kit; SIS alpha ELISA Kit; SIS beta ELISA Kit; SIS-beta ELISA Kit; Small inducible cytokine A3 ELISA Kit; small inducible cytokine A3 (homologous to mouse Mip-1a) ELISA Kit; Small-inducible cytokine A3 ELISA Kit; Tonsillar lymphocyte LD78 alpha protein ELISA Kit |
| Abbreviation | CCL3 |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 4.69 pg/mL-300 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 93; 1: Range %; 2: 89-98; 1: 1:2; 2: Average %; 3: 90; 1: Range %; 2: 85-96; 1: 1:4; 2: Average %; 3: 98; 1: Range %; 2: 91-104; 1: 1:8; 2: Average %; 3: 89; 1: Range %; 2: 82-96 |
| Recovery | headers: The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 92; 3: 85-99; 1: EDTA plasma (n=4); 2: 97; 3: 90-105 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 300; 2: 2.105; 3: 2.184; 4: 2.145; 5: 2.002; 1: 150; 2: 1.814; 3: 1.787; 4: 1.801; 5: 1.658; 1: 75; 2: 1.375; 3: 1.288; 4: 1.331; 5: 1.189; 1: 37.5; 2: 0.705; 3: 0.709; 4: 0.707; 5: 0.565; 1: 18.75; 2: 0.463; 3: 0.461; 4: 0.462; 5: 0.320; 1: 9.4; 2: 0.237; 3: 0.253; 4: 0.245; 5: 0.103; 1: 4.7; 2: 0.220; 3: 0.218; 4: 0.219; 5: 0.077; 1: 0; 2: 0.144; 3: 0.141; 4: 0.142 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This ELISA kit provides quantitative measurement of CCL3 (MIP-1α) in human samples, supporting research into innate immune activation, inflammatory signaling, and leukocyte chemotaxis. Because CCL3 is rapidly secreted at low concentrations by activated macrophages and T cells during early inflammatory responses, detecting it requires an assay calibrated for the picogram-level range typical of clinical and in vitro specimens. Detection Range: The 4.69–300 pg/mL range aligns with circulating CCL3 levels observed in healthy serum (typically <50 pg/mL) and the elevated concentrations reported in sepsis, HIV infection, and rheumatoid arthritis synovial fluid. Most serum and plasma samples can be assayed neat or with minimal dilution, while stimulated cell culture supernatants may require dilution into range. Sensitivity: At 1.17 pg/mL, the assay detects CCL3 in low-abundance contexts such as unstimulated baseline plasma, cerebrospinal fluid, or early time points in macrophage activation kinetics where secretion is just initiating. Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables paired designs comparing systemic CCL3 levels with local tissue expression—critical for studies dissecting compartmentalized inflammation in joints, tumors, or lung tissue. Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate limited clinical specimens and time-course experiments requiring multiple sampling from primary monocyte or PBMC cultures. This kit is well-suited for studies in sepsis biomarker profiling, tumor microenvironment characterization, and chronic inflammatory disease monitoring. |
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
