Human Retinoic Acid ELISA Kit CSB-E16712h CUSABIO
$50.00
In stock
SKU
CSB-E16712h
Product Details
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.12 pg/ml |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Abbreviation | Retinoic Acid |
| Sample Types | serum, plasma |
| Detection Range | 6.25 pg/ml-100 pg/ml |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Epigenetics and Nuclear Signaling |
| Assay Principle | quantitative |
| Measurement | Competitive |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 93; 1: Range %; 2: 86-100; 1: 1:200; 2: Average %; 3: 88; 1: Range %; 2: 81-95; 1: 1:400; 2: Average %; 3: 96; 1: Range %; 2: 89-102; 1: 1:800; 2: Average %; 3: 85; 1: Range %; 2: 81-90 |
| Recovery | headers: The recovery of human retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 90; 3: 83-97; 1: EDTA plasma (n=4); 2: 87; 3: 82-94 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 1: 100; 2: 0.146; 3: 0.138; 4: 0.142; 1: 50; 2: 0.325; 3: 0.337; 4: 0.331; 1: 25; 2: 0.649; 3: 0.618; 4: 0.634; 1: 12.5; 2: 1.123; 3: 1.059; 4: 1.091; 1: 6.25; 2: 1.685; 3: 1.601; 4: 1.643; 1: 0; 2: 2.096; 3: 2.103; 4: 2.100 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Retinoic acid is a bioactive metabolite of vitamin A that plays important roles in cellular differentiation, embryonic development, and immune system regulation. This small molecule acts as a ligand for nuclear retinoic acid receptors, influencing gene transcription and cellular processes including apoptosis, proliferation, and tissue homeostasis. Retinoic acid signaling pathways are necessary for proper organ formation during embryogenesis. They also continue to regulate adult tissue maintenance and repair mechanisms. The Human Retinoic Acid ELISA Kit (CSB-E16712h) provides quantitative measurement of retinoic acid in human samples using a competitive assay principle. This kit requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection is performed at 450 nm wavelength, allowing researchers to analyze retinoic acid levels in various biological specimens from Homo sapiens samples. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This Human Retinoic Acid ELISA Kit has been used in diverse research applications focused on cellular metabolism, vitamin A pathway analysis, and immune system studies. The kit allows quantitative measurement of retinoic acid levels in cell lysates and biological samples to study metabolic processes and cellular responses. Research applications span from basic cellular biology to infection-related immune responses. • Cellular metabolism research: Analysis of retinol dehydrogenase activity and vitamin A metabolic pathway analysis in cultured cells • Intracellular signaling studies: Quantification of retinoic acid levels in cells with altered protein expression to understand metabolic regulation • Infection and immunity research: Study of retinoic acid levels in the context of bacterial infections and immune cell responses • Biomarker analysis: Measurement of retinoic acid concentrations as part of broader metabolic and inflammatory marker profiling |
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