Human defensin, alpha 1 (DEFA1) ELISA kit CSB-E14155h CUSABIO
$50.00
In stock
SKU
CSB-E14155h
Product Details
| Target Name | defensin, alpha 1 |
| Uniprot No. | P59665 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.156 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | alpha 1 ELISA Kit; DEF1 ELISA Kit; DEF1_HUMAN ELISA Kit; DEFA1 ELISA Kit; DEFA1B ELISA Kit; DEFA2 ELISA Kit; Defensin 1 ELISA Kit; Defensin ELISA Kit; Defensin; alpha 1 ELISA Kit; Defensin; alpha 1; myeloid related sequence ELISA Kit; Defensin; alpha 2 ELISA Kit; HNP-1 ELISA Kit; HNP-2 ELISA Kit; HNP1 ELISA Kit; HP-1 ELISA Kit; HP-2 ELISA Kit; HP1 ELISA Kit; HP2 ELISA Kit; MRS ELISA Kit; Myeloid related sequence ELISA Kit; Neutrophil defensin 1 ELISA Kit; Neutrophil defensin 2 ELISA Kit |
| Abbreviation | DEFA1 |
| Sample Types | serum, plasma, urine |
| Detection Range | 0.625 ng/mL-40 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 89; 1: Range %; 2: 83-94; 1: 1:2; 2: Average %; 3: 84; 1: Range %; 2: 80-88; 1: 1:4; 2: Average %; 3: 93; 1: Range %; 2: 89-97; 1: 1:8; 2: Average %; 3: 95; 1: Range %; 2: 90-99 |
| Recovery | headers: The recovery of human DEFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human DEFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human DEFA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 97; 3: 94-102; 1: EDTA plasma (n=4); 2: 90; 3: 86-96; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 40 2.058 2.014 2.036 1.933 20 1.547 1.525 1.536 1.433 10 1.104 1.071 1.088 0.985 5 0.673 0.715 0.694 0.591 2.5 0.459 0.437 0.448 0.345 1.25 0.309 0.312 0.311 0.208 0.625 0.205 0.194 0.200 0.097 0 0.107 0.099 0.103; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 40; 9: 2.058; 10: 2.014; 11: 2.036; 12: 1.933; 14: 20; 15: 1.547; 16: 1.525; 17: 1.536; 18: 1.433; 20: 10; 21: 1.104; 22: 1.071; 23: 1.088; 24: 0.985; 26: 5; 27: 0.673; 28: 0.715; 29: 0.694; 30: 0.591; 32: 2.5; 33: 0.459; 34: 0.437; 35: 0.448; 36: 0.345; 38: 1.25; 39: 0.309; 40: 0.312; 41: 0.311; 42: 0.208; 44: 0.625; 45: 0.205; 46: 0.194; 47: 0.200; 48: 0.097; 50: 0; 51: 0.107; 52: 0.099; 53: 0.103; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 40; 2: 2.058; 3: 2.014; 4: 2.036; 5: 1.933; 1: 20; 2: 1.547; 3: 1.525; 4: 1.536; 5: 1.433; 1: 10; 2: 1.104; 3: 1.071; 4: 1.088; 5: 0.985; 1: 5; 2: 0.673; 3: 0.715; 4: 0.694; 5: 0.591; 1: 2.5; 2: 0.459; 3: 0.437; 4: 0.448; 5: 0.345; 1: 1.25; 2: 0.309; 3: 0.312; 4: 0.311; 5: 0.208; 1: 0.625; 2: 0.205; 3: 0.194; 4: 0.200; 5: 0.097; 1: 0; 2: 0.107; 3: 0.099; 4: 0.103 |
| Materials provided | A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human DEFA1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates. Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve. One vial Biotin-labeled DEFA1antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody. One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic. One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody. One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration. One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances. One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue. One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow. Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation. An instruction manual |
| Materials not provided | A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. An incubator can provide stable incubation conditions up to 37°C±5°C. Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate 50-300ul multi-channel micropipette Pipette tips Single-channel micropipette with different ranges 100ml and 500ml graduated cylinders Deionized or distilled water Timer Test tubes for dilution |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Defensin alpha 1 (DEFA1) is an antimicrobial peptide belonging to the alpha-defensin family, primarily produced by neutrophils and Paneth cells in the small intestine. This cationic peptide functions in innate immunity by disrupting microbial cell membranes and showing broad-spectrum antimicrobial activity against bacteria, fungi, and enveloped viruses. DEFA1 is stored in neutrophil granules and released upon activation, contributing to the first line of defense against pathogens. Research has linked DEFA1 to various inflammatory conditions and immune responses, making it an important biomarker for studying innate immunity and host-pathogen interactions. The Human defensin, alpha 1 (DEFA1) ELISA kit (CSB-E14155h) is a quantitative sandwich assay designed for measuring DEFA1 levels in human serum, plasma, and urine samples. The kit has a detection range of 0.625 ng/mL to 40 ng/mL with a sensitivity of 0.156 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. This provides researchers with a reliable tool for quantifying this antimicrobial peptide in various biological specimens. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in clinical research studies to quantify defensin alpha 1 levels in human biological samples. The kit supports comparative analyses between patient populations and healthy controls, as well as biomarker validation studies in cardiovascular and infectious disease research. • Cardiovascular research: Used to validate defensin alpha 1 as a potential biomarker in acute myocardial infarction studies, comparing plasma levels between patients and healthy controls • Infectious disease studies: Applied in COVID-19 research to measure serum defensin alpha 1 concentrations and assess differences between infected patients and control groups • Biomarker validation: Used in multi-protein panels to confirm proteomic findings and validate differentially expressed proteins identified through discovery-phase analyses • Clinical sample analysis: Supports quantitative measurement of defensin alpha 1 in human serum and plasma samples for disease-related biomarker studies |
MDSD
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
