Human mammalian target of rapamyein,mTOR ELISA Kit CSB-E09038h CUSABIO
$50.00
In stock
SKU
CSB-E09038h
Product Details
| Target Name | mammalian target of rapamyein,mTOR |
| Uniprot No. | P42345 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.39 pg/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) ELISA Kit; FK506 binding protein 12 rapamycin associated protein 1 ELISA Kit; FK506 binding protein 12 rapamycin associated protein 2 ELISA Kit; FK506 binding protein 12 rapamycin complex associated protein 1 ELISA Kit; FK506-binding protein 12-rapamycin complex-associated protein 1 ELISA Kit; FKBP rapamycin associated protein ELISA Kit; FKBP12 rapamycin complex associated protein ELISA Kit; FKBP12-rapamycin complex-associated protein 1 ELISA Kit; FKBP12-rapamycin complex-associated protein ELISA Kit; FLJ44809 ELISA Kit; FRAP ELISA Kit; FRAP1 ELISA Kit; FRAP2 ELISA Kit; Mammalian target of rapamycin ELISA Kit; Mechanistic target of rapamycin ELISA Kit; mTOR ELISA Kit; MTOR_HUMAN ELISA Kit; OTTHUMP00000001983 ELISA Kit; RAFT1 ELISA Kit; Rapamycin and FKBP12 target 1 ELISA Kit; Rapamycin associated protein FRAP2 ELISA Kit; Rapamycin target protein 1 ELISA Kit; Rapamycin target protein ELISA Kit; RAPT1 ELISA Kit; Serine/threonine-protein kinase mTOR ELISA Kit |
| Abbreviation | mTOR |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 1.56 pg/mL-100 pg/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Cell Biology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human mTOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human mTOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human mTOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:100; 2: Average %; 3: 86; 1: Range %; 2: 80-92; 1: 1:200; 2: Average %; 3: 98; 1: Range %; 2: 91-105; 1: 1:400; 2: Average %; 3: 101; 1: Range %; 2: 92-110; 1: 1:800; 2: Average %; 3: 93; 1: Range %; 2: 86-98 |
| Recovery | headers: The recovery of human mTOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human mTOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human mTOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 93; 3: 89-98; 1: EDTA plasma (n=4); 2: 97; 3: 90-100; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: pg/ml OD1 OD2 Average Corrected 100 2.675 2.666 2.671 2.546 50 2.312 2.189 2.251 2.126 25 1.709 1.663 1.686 1.561 12.5 1.047 0.989 1.018 0.893 6.25 0.617 0.603 0.610 0.485 3.12 0.429 0.412 0.421 0.296 1.56 0.238 0.229 0.234 0.109 0 0.129 0.121 0.125; 2: pg/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 100; 9: 2.675; 10: 2.666; 11: 2.671; 12: 2.546; 14: 50; 15: 2.312; 16: 2.189; 17: 2.251; 18: 2.126; 20: 25; 21: 1.709; 22: 1.663; 23: 1.686; 24: 1.561; 26: 12.5; 27: 1.047; 28: 0.989; 29: 1.018; 30: 0.893; 32: 6.25; 33: 0.617; 34: 0.603; 35: 0.610; 36: 0.485; 38: 3.12; 39: 0.429; 40: 0.412; 41: 0.421; 42: 0.296; 44: 1.56; 45: 0.238; 46: 0.229; 47: 0.234; 48: 0.109; 50: 0; 51: 0.129; 52: 0.121; 53: 0.125; 1: pg/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 100; 2: 2.675; 3: 2.666; 4: 2.671; 5: 2.546; 1: 50; 2: 2.312; 3: 2.189; 4: 2.251; 5: 2.126; 1: 25; 2: 1.709; 3: 1.663; 4: 1.686; 5: 1.561; 1: 12.5; 2: 1.047; 3: 0.989; 4: 1.018; 5: 0.893; 1: 6.25; 2: 0.617; 3: 0.603; 4: 0.610; 5: 0.485; 1: 3.12; 2: 0.429; 3: 0.412; 4: 0.421; 5: 0.296; 1: 1.56; 2: 0.238; 3: 0.229; 4: 0.234; 5: 0.109; 1: 0; 2: 0.129; 3: 0.121; 4: 0.125 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | This Human mTOR ELISA Kit was designed for the quantitative measurement of Human mTOR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL. |
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