Human myosin light chain kinase,MLCK ELISA Kit CSB-E09048h CUSABIO
$50.00
In stock
SKU
CSB-E09048h
Product Details
| Target Name | myosin light chain kinase |
| Uniprot No. | Q15746 |
| Species | Homo sapiens (Human) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 0.195 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | deglutamylated form ELISA Kit; DKFZp686I10125 ELISA Kit; EC 2.7.11.18 ELISA Kit; FLJ12216 ELISA Kit; Kinase related protein ELISA Kit; Kinase-related protein ELISA Kit; KRP ELISA Kit; MLCK ELISA Kit; MLCK1 ELISA Kit; MLCK108 ELISA Kit; MLCK210 ELISA Kit; MSTP083 ELISA Kit; MYLK ELISA Kit; MYLK_HUMAN ELISA Kit; MYLK1 ELISA Kit; Myosin light chain kinase ELISA Kit; Myosin light polypeptide kinase ELISA Kit; OTTHUMP00000180642 ELISA Kit; OTTHUMP00000180643 ELISA Kit; smMLCK ELISA Kit; smooth muscle ELISA Kit; Smooth muscle myosin light chain kinase ELISA Kit; Telokin ELISA Kit |
| Abbreviation | MYLK |
| Sample Types | serum, plasma, tissue homogenates, cell lysates |
| Detection Range | 0.78 ng/mL-50 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess. |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of human MLCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of human MLCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of human MLCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 82; 1: Range %; 2: 80-85; 1: 1:2; 2: Average %; 3: 97; 1: Range %; 2: 94-100; 1: 1:4; 2: Average %; 3: 87; 1: Range %; 2: 82-92; 1: 1:8; 2: Average %; 3: 97; 1: Range %; 2: 91-103 |
| Recovery | headers: The recovery of human MLCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of human MLCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of human MLCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 86-91; 1: EDTA plasma (n=4); 2: 104; 3: 100-108 |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; ; ; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 50; 2: 2.420; 3: 2.468; 4: 2.444; 5: 2.302; 1: 25; 2: 1.957; 3: 1.969; 4: 1.963; 5: 1.821; 1: 12.5; 2: 1.298; 3: 1.279; 4: 1.289; 5: 1.147; 1: 6.25; 2: 0.764; 3: 0.728; 4: 0.746; 5: 0.604; 1: 3.12; 2: 0.368; 3: 0.352; 4: 0.360; 5: 0.218; 1: 1.56; 2: 0.288; 3: 0.298; 4: 0.293; 5: 0.151; 1: 0.78; 2: 0.202; 3: 0.204; 4: 0.203; 5: 0.061; 1: 0; 2: 0.140; 3: 0.144; 4: 0.142 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | The human myosin light chain kinase (MLCK) Elisa kit is suitable for the quantitative measurement of human MLCK in serum, plasma, cell lysates, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of MLCK in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, and lot-to-lot consistency. MLCK (MYLK) is a Ca 2+ /CaM-dependent Ser/Thr-kinase that phosphorylates the regulatory light chain (RLC) of the motor protein myosin-II. MLCK plays an important role in maintaining the equilibrium by phosphorylating myosin light chain (MLC), thereby inducing actomyosin contractility and weakening endothelial cell–cell adhesion. MLCK plays numerous roles in regulating cellular function based on the multi-faceted role of myosin in cell biology. MLCK is activated by numerous physiological factors and inflammatory or angiogenic mediators, causing vascular hyperpermeability. MLCK functions in cytoskeleton organization, cytokinesis, and aggregation in a kinase-independent manner. |
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